100(P-34) 熱帯性毒きのこオオシロカラカサタケ(Chlorophyllum molybdites)の毒成分研究 : 毒性タンパク質molybdophyllysinの構造とメタロエンドペプチダーゼとしての酵素的性質(ポスター発表の部)

概要

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The toadstool Chlorophyllum molybdites (one of the previous names, Lepiota morganii) is common in the tropics, and its poisoning has been known for more than 100 years. In recent years, however, this fungus has become widely distributed in Japan probably due to the global climate change, which resulted in the increase of this mushroom poisoning in this country. Although the poisoning is rarely fatal, the symptoms are severe: that is, vomiting, diarrhea, chill, intestinal pain, stomachache, and in some cases convulsions. Early studies on the toxic components of this fungus suggested the presence of some cholinergic compounds. However, Eilers and Nelson later demonstrated that the toxic principle was proteinaceous, and reported partial purification and characterization of a toxic protein. Isolation of non-toxic alkaloids and cytotoxic steroids were also reported recently. The recent increase of this mushroom poisoning prompted us to reinvestigate the toxic components of this fungus guided by the lethal effect on mice, which led to the isolation of a protein, named molybdophyllysin. This toxic protein turned out to be highly homologous to metalloendopeptidases previously found from edible mushrooms, such as Grifola frondosa, Pleurotus ostreatus, and Armillaria mellea. Here we report the isolation of molybdophyllysin, its partial amino acid sequences, and enzymatic properties.
天然有機化合物討論会の論文
2004-10-01