72. Arabidopsis thalianaを用いたα-ketol linolenic acid(KODA)の生理作用とマイクロアレイを用いた遺伝子発現解析(口頭発表)

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Oxylipin, 9-hydroxy-10-oxo-12(Z), 15(Z)-octadecadienoic acid (KODA) is a signal compound found mLemunapaucicostata after exposure to stress, such as drought, heat or osmotic stress. According to the subsequent research, it has reported that the flower-inducing activities were found in Lemuna paucicostata and Pharbitis nil (violet). To understand function of KODA in Arabidopsis thaliana (L.) ecotype Coumbia, we performed phylogenetic analysis. First, we measured endogenous KODA levels in Arabisopsis to confirm their existences. The 145pg/g-F.W KODA was certanly detected. Next, to detect KODA's function, we sprayed KODA to seedlings. But under the continuous light condition, none of the KODA's influence was detected. However some morphological changes were induced when the 10μM KODA were treated under stress condition. Under the short day condition (7h light-17h dark), both Rosette leaf enlargement and acceleration of lateral buds formation were detected from the late-vegetative to early-reproductive stages. Under continuous light condition with high-Nitrogen fertilizer treatment, not only leaf enlargement but also vigorous inflorescences development was detected. Next, to detect KODA's effect distinctly, we sprayed KODA to the mutant aos whitch is the half concentration of KODA compared to that of WT. In aos, lateral root formation was accerelated by the KODA treatment only grown in soil. To detect KODA induced gene expression, the seedlings were cut into two parts. And Microarray analysis were occupied. KODA did not induce organogenesis-related-genes expression but induced some plant-hormonerelated gene expressions.
植物化学調節学会の論文
2007-10-05

72. Arabidopsis thalianaを用いたα-ketol linolenic acid(KODA)の生理作用とマイクロアレイを用いた遺伝子発現解析(口頭発表)

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