Polysaccharides in Fungi. XX. Structure and Antitumor Activity of a Branched (1→3)-β-D-Glucan from Alkaline Extract of Yu er(Biological,Chemical)

概要

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A water-insoluble glucan (N-5P), [α]^<23>_D + 2.3° (c=0.51, 0.5M sodium hydroxide), was isolated from the alkaline extract of the fruit bodies of Yu er (Chinese name) (Auricularia species). N-5P was homogeneous as judged by gel filtration and electrophoresis. By gel filtration, the molecular weight of N-5P was estimated to be 5.6×10^5. The ^<13>C-nuclear magnetic resonance and infrared spectra, together with the result of chromium trioxide oxidation, indicated that glucosidic linkages in N-5P have the β-D configuration. From the results of methylation analysis, periodate oxidation, Smith degradation (complete, as well as partial), partial acetolysis, and enzymic hydrolysis, it was concluded that N-5P has a main chain composed of β-(1→3)-linked D-glucopyranosyl residues, with single, β-(1→6)-linked D-glucopyranosyl groups attached as side chains, which account for a quarter of the total sugar residues. In addition, the results of enzymic hydrolysis indicated that the β-(1→6)-linked D-glucosyl side chains are mainly localized in the neighborhood of the nonreducing end of the main chain. Furthermore, N-5P showed potent antitumor activity against the solid form of sarcoma 180 in mice, and exhibited significant carbon clearance-enhancing activity in mice.
社団法人日本薬学会の論文
1987-10-25