Human Type II GnRH Receptor Mediates Effects of GnRH on Cell Proliferation(Endocrinology)
スポンサーリンク
概要
- 論文の詳細を見る
GnRH (gonadotropin-releasing hormone) is well-known as the central regulator of the reproductive system through its stimulation of gonadotropin release from the pituitary. Progress in studies on GnRH demonstrated that GnRH has both inhibitory and stimulatory effects on cell proliferation depending on the cell type, and the mechanisms of these effects have been intensively studied. However, even human GnRH receptors which mediate GnRH stimulation have not been complately identified. In the present study, we showed that the inhibitory and stimulatory effects of GnRH on colony-formation using four cell lines and have demonstrated that the inhibitory and stimulatory effects of GnRH exhibit distinctly different patterns of ligand sensitivity. This result strongly suggests that the two opposite effects of GnRH occur via different types of GnRH receptors, however expressional analyses os human GnRH receptors did not exhibit the significantly different pattern between negatively and positively responding cell lines. Then, in order to identify the GnRH receptors involved in the two opposite effects, errects of GnRH were analysed under the conditions that human GnRH receptors were knocked down by the technique of RNA interference. Consequently, it was found that human type II GnRH receptor mediates GnRH simulation and its splice variant determines the direction of the response to GnRH.These results are the first clear evidence for the functionality of human type II GnRH receptor. Therefore our novel findings are quite noticeable and will greatly contribute to the studies on the mechanisms of the effects of GnRH on cell proliferation in the future.
- 社団法人日本動物学会の論文
- 2004-01-25
著者
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Park Min
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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ENDO Daisuke
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
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Enomoto Masahiro
Department of Biological Sciences, Graduate School of Science, The University of Tokyo
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Kawashima Seiichiro
Research Laboratory, Zenyaku Kogyo Co.,Ltd.
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Park M
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Endo Daisuke
Department Of Biological Science Graduate School Of Science The University Of Tokyo
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Enomoto Masahiro
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Enomoto Masahiro
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Kawashima Seiichiro
Research Laboratory Zenyaku Kogyo Co. Ltd.
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Kawashima Seiichiro
Department Of Biosciences Research Laboratory Zenyaku Kogyo Co. Ltd.
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Enomoto M
Univ. Tokyo Tokyo Jpn
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Kawashima S
Research Laboratory Zenyaku Kogyo Co. Ltd.
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Park Min
Department Of Biological Science Graduate School Of Science The University Of Tokyo
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Endo Daisuke
Department Of Animal Science Tokyo University Of Agriculture
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Park Min
Department of Applied Sciences, Korea Maritime University, Busan 606-791, Korea
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