20(S)-Ginsenoside Rg3 Enhances Glucose-Stimulated Insulin Secretion and Activates AMPK(Pharmacology)
スポンサーリンク
概要
- 論文の詳細を見る
Although Panax ginseng has been widely used in oriental countries for pharmacological effects such as anti-diabetic, anti-inflammatory, adaptogenic and anti-fatigue activities, the active ingredient is not yet fully identified. In our preliminary studies, protopanaxadiol ginsenosides showed the insulin secretion-stimulating activity. In HIT-T15 cells, Rg3 enhanced the insulin secretion in a concentration dependent manner. This effect, however, was almost completely abolished in the presence of diazoxide (K^+ channel opener) or nifedipine (Ca^<2+> channel blocker). Oral glucose tolerance test (OGTT) was also performed using ICR mice and Rg3 suppressed the blood glucose levels from rising by enhancing an insulin secretion at 30 min after administration. From these studies, we may conclude that Rg3 lowered the plasma glucose level by stimulating an insulin secretion and this action was presumably associated with ATP sensitive K^+ channel. Next, to explore the hypothesis that ginsenoside Rg3 epimers may exhibit differential effects, glucose-stimulated insulin secretion activity and phosphorylation of AMP-activated protein kinase (AMPK) were compared between 20(S)- and 20(R)-ginsenoside Rg3. 5 μM of 20(S)-Rg3 enhanced the glucose-stimulated insulin secretion by 58% compared to the control, but 20(R)-Rg3 did not show any effect. In C2C12 myotubes, 20(S)- and 20(R)-Rg3 both markedly phosphorylated AMPK and acetyl-CoA carboxylase (ACC), although 20(R)-Rg3 showed a little less effect. Taken together, our results suggest that ginsenoside Rg3 epimers showed differential activities, and 20(S)-Rg3 epimer exhibited the higher pharmacological effects in insulin secretion and AMPK activation than 20(R)-Rg3. The novel characteristics of 20(S)-Rg3 may be a valuable candidate for anti-diabetic agent.
- 2008-04-01
著者
-
Park Min
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
-
Chung Sung
Department Of Life And Nanopharmaceutical And Department Of Pharmaceutical Science Kyung Hee Univers
-
Ha Joohun
Department Of Biochemistry And Molecular Biology Kyung Hee University School Of Medicine
-
Park Min
Department Of Biological Science Graduate School Of Science The University Of Tokyo
-
Park Min
Department Of Life And Nanopharmaceutical And Department Of Pharmaceutical Science Kyung Hee Univers
-
Chung Sung
Department Of Anesthesiology And Pain Medicine
-
Park Min
Department of Applied Sciences, Korea Maritime University, Busan 606-791, Korea
関連論文
- ANALYSIS OF THE RELATIONSHIP BETWEEN THE EXPRESSIONS OF AD4BP/SF-1 AND STEROIDOGENIC ENZYMES IN THE DEVELOPING GONADS OF A SQUAMATA SPECIES WITH TEMPERATURE-DEPENDENT SEX DETERMINATION
- Molecular Characterization of Thyroid Hormone Receptors from the Leopard Gecko, and Their Differential Expression in the Skin(Endocrinology)
- IDENTIFICATION OF TWO SUBTYPES OF THYROID HORMONE RECEPTOR cDNAs IN THE LEOPARD GECKO, EUBLEPHARIS MACULARIUS(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- EXPRESSION OF GnRH RECEPTOR GENES IN THE DWARF GOURAMI (COLISA LALIA) BRAIN AS REVEALED BY IN SITU HYBRIDIZATION(Physiology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Human Type II GnRH Receptor Mediates Effects of GnRH on Cell Proliferation(Endocrinology)
- CLONING AND CHARACTERIZAION OF A GUINEA PIG GnRH RECEPTOR(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- The Difference in Autofluorescence Features of Lipofuscin between Brain and Adrenal
- Development of Constitutive Equation on Superplastic RS P/M Mg-Y-Zn Alloy
- Low-Threshold-Current and Single-Mode Surface-Emitting Laser Buried in Amorphous GaAs
- Low Threshold Current Density Surface-Emitting Lasers Buried by Amorphous GaAs
- Ginsenoside Rg1 Suppresses Hepatic Glucose Production via AMP-Activated Protein Kinase in HepG2 Cells
- MOLECULAR EVOLUTION OF PREPRO-THYROTROPIN-RELEASING HORMONE
- THE FUNCTIONAL ANALYSIS OF THE TRANSCRIPTS DERIVED FROM HUMAN TYPE II GNRH RECEPTOR GENE
- THE ROLE OF HUMAN TYPE II GNRH RECEPTOR GENE IN THE REGULATION OF CELL PROLIFERATION
- Peroxisome Proliferator-activated Receptor Gamma Activation Induces Cell Cycle Arrest via the p53-independent Pathway in Human Anaplastic Thyroid Cancer Cells
- STIMULATORY AND INHIBITORY REGULATION OF GnRH ON THE PROLIFERATION OF CELLS WITH DIFFERENT LIGAND SENSITIVITIES
- Polarization Properties of Vertical-Cavity Surface-Emitting Lasers with Various Patterns in Tilted Pillar Structures
- Suppression of Macroscopic Defects through the Control of Friction between Workpiece and Dies in a Forged Magnesium Alloy Part with Ribs
- 20(S)-Ginsenoside Rg3 Enhances Glucose-Stimulated Insulin Secretion and Activates AMPK(Pharmacology)
- Her2^ genotype and breast cancer progression in Korean women
- Creep Deformation Mechanisms in Coarse-Grained Solid Solution Mg Alloys
- The Effect of Ram Speed on Mechanical and Thermal Properties in ECAE Process Simulation
- Protective Effects of an Extract of Young Radish (Raphanus sativus L) Cultivated with Sulfur (Sulfur-Radish Extract) and of Sulforaphane on Carbon Tetrachloride-Induced Hepatotoxicity
- EXPRESSIONAL CHARACTERIZATION OF TWO TYPES OF GnRH RECEPTOR IN A REPTILIAN SPECIES, LEOPARD GECKO
- GnRH AFFECTS COLONY FORMATION, REGULATING CELL PROLIFERATION
- THE ESTABLISHMENT OF GnRH RECEPTOR KNOCKED-DOWN CELL LINES; AN APPROACH TO THE ROLE OF GnRH RECEPTOR IN THE REGULATION OF CELL PROLIFERATION BY GnRH(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Jap
- REGULATIONS AND MOLECULAR MECHANISIMS OF CELL PROLIFERATION AND CELL MIGRATION BY GONADOTROPIN-RELEASING HORMONE
- Stimulatory and Inhibitory Regulation of Gnrh on Cell Proliferation : Involvement of Different Gnrh Receptor Subtypes(Multidisciplinary Search for the Secrets of Eleven-Headed Kannon-Like Biological Functions of Peptide,Symposium,PROCEEDING OF THE 75^
- THE EFFECT OF INCUBATION TEMPERATURE ON THE SEX STEROID HORMONE SIGNALING SYSTEMS IN THE BRAIN AND GONAD OF THE SQUAMATA SPECIES WITH TEMPERATURE-DEPENDENT SEX DETERMINATION
- THE EXPRESSION ANALYSIS OF DMRT1 mRNA IN THE DIFFERENTIATING TESTIS OF THE LEOPARD GECKO, SQUAMATA SPECIES WITH TEMPERATURE-DEPENDENT SEX DETERMINATION
- IDENTIFICATION OF THE P450AROMATASE cDNA FROM THE LEOPARD GECKO, A LIZARD WITH TEMPERATURE-DEPENDENT SEX DETERMINATION AND ITS EXPRESSION IN THE OVARY(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of J
- ANALYSIS OF mRNA EXPRESSION LEVELS OF THREE SEX STEROID RECEPTORS IN VARIOUS ORGANS OF THE LEOPARD GECKO, A LIZARD WITH TEMPERATURE-DEPENDENT SEX DETERMINATION
- Drosophila Calpain B is Monomeric and Autolyzes Intramolecularly
- Protective Effects of an Extract of Young Radish (Raphanus sativus L) Cultivated with Sulfur (Sulfur-Radish Extract) and of Sulforaphane on Carbon Tetrachloride-Induced Hepatotoxicity
- Ontogeny of the Expression of Messenger Ribonucleic Acid Encoding Lutropin Receptor in Chicken Embryo
- COMPARATIVE ANALYSIS OF THE GONADAL AND PITUITARY GNRH SYSTEMS IN CHORDATES
- MOLECULAR CHARACTERIZATION OF MULTIPLE TRANSCRIPTS FROM TWO DISTINCT GnRHR GENES IN THE LEOPARD GECKO
- GnRH as a Cell Proliferation Regulator : Mechanism of Action and Evolutionary Implications
- STIMULATORY AND INHIBITORY REGULATION OF GnRH ON CELL PROLIFERATION OF HUMAN PROSTATIC CARCINOMA CELL LINES, TSU-Pr1 AND DU145
- Effects of Photowashing Treatment on Gate Leakage Current of GaAs Metal-Semiconductor Field-Effect Transistors
- COMPARATIVE ANALYSIS OF THE PITUITARY AND GONADAL GNRH SYSTEMS IN THE LEOPARD GECKO
- Chicken RFamide-related Peptide (GnIH) and Two Distinct Receptor Subtypes : Identification, Molecular Characterization, and Evolutionary Considerations
- IDENTIFICATION OF THE CHICKEN GnRH-II GENE IN THE CHICKEN AND CHARACTERIZATION OF ITS DISTINCTIVE GENOMIC ORGANIZATION AND EXPRESSION PATTERN(Endocrinology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Difficulties at work experienced by patients with inflammatory bowel disease (IBD) and factors relevant to work motivation and depression
- THREE GnRHs AND FIVE GnRH RECEPTORS IN THE SPOTTED GREEN PUFFERFISH TETRAODON NIGROVIRIDIS AND A NOVEL CLASSIFICATION OF VERTEBRATE GnRH RECEPTORS(Biochemistry & Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological S
- Hemostatic and Electrolyte Effects of Hydroxyethyl Starches in Patients Undergoing Posterior Lumbar Interbody Fusion Using Pedicle Screws and Cages
- ニワトリのRFアミド関連ペプチド(GnIH)と異なる2種類の受容体:同定・特性解析・進化学的考察
- Evaporation Rate of Free Paraffin Hydrocarbon Droplets in a High-Temperature and High-Pressure Gas Stream
- MIGRATION OF GnRH NEURONS IN THREE-DIMENSIONAL CULTURE OF EMBRYONIC CHICK OLFACTORY NERVE BUNDLE
- GnRH RECEPTORS AND LIGANDS IN TELEOST TN-GnRH NEURONS(Physiology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- GONADOTROPIN-RELEASING HORMONE RECEPTOR mRNA EXPRESSION IN THE OVARIES OF NEONATAL AND ADULT RATS
- Changes in the Expression Pattern of Luteinizing Hormone Receptor mRNA in Rat Testis during Degeneration of Seminiferous Epitheliun
- EXPRESSIONAL ANALYSIS OF ESTROGENIC ENZYME AND ESTROGEN RECEPTORS IN THE KIDNEYS OF THE LEOPARD GECKO, EUBLEPHARIS MACULRIUS(Endocrinology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- HLA-DR Antigens in Japanese with Hashimotos Thyroiditis and Graves Disease
- Modified Julolidine-Containing Emitters for Red Organic Light-Emitting Diodes
- EXPRESSION ANALYSIS OF BDNF IN THE VENTROMEDIAL HYPOTHALAMIC NUCLEUS OF THE CHICK EMBRYO
- MOLECULAR IDENTIFICATIONS OF THREE ISOFORMS OF PPARS (PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORS) IN THE LEOPARD GECKO, Eublepharis macularius
- MOLECULAR IDENTIFICATION OF PPARγ (PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA) IN THE LEOPARD GECKO, EUBLEPHARIS MACULARIUS
- IDENTIFICATION OF THE REPTILIAN UNCOUPLING PROTEINS IN THE LEOPARD GECKO, Eublepharis macularius
- MOLECULAR CLONING OF PRO-OPIOMERANOCORTIN GENE AND ITS TISSUE DISTRIBUTION IN THE LEOPARD GECKO, Eublepharis macularius
- Effect of Tranexamic Acid on Transfusion Requirement in Dual Antiplatelet-Treated Anemic Patients Undergoing Off-Pump Coronary Artery Bypass Graft Surgery : A Randomized Controlled Study
- ANALYSIS OF THE EXPRESSION OF GnRH RECEPTOR mRNA IN THE RAT TESTIS
- Safety and Effect of Adipose Tissue-Derived Stem Cell Implantation in Patients With Critical Limb Ischemia : A Pilot Study
- Validation of Intra-Subject Variation in Biodynamic Responses of Seated Human Exposed to Whole-Body Vibration
- Entanglement Perturbation Theory for Antiferromagnetic Heisenberg Spin Chains
- Low-Threshold-Current and Single-Mode Surface-Emitting Laser Buried in Amorphous GaAs
- Variation of Floor-to-Head Transmissibility in Seated Human during Prolonged Driving Simulation
- Change of Seat-to-Head Transmissibilities at Two-Hour Intervals with and without Whole-Body Vibration
- Effect of NaF and CaO/SiO2 on the Hydrogen Dissolution in the CaO–SiO2–FeOt Based Flux System
- Growth of GaN on Metallic Compound Graphite Substrate Using Hydride Vapor Phase Epitaxy
- Eugenol Ameliorates Hepatic Steatosis and Fibrosis by Down-Regulating SREBP1 Gene Expression via AMPK-mTOR-p70S6K Signaling Pathway
- Effect of NaF and CaO/SiO_2 on the Hydrogen Dissolution in the CaO-SiO_2-FeO_t Based Flux System