Micro-radioreceptor Assay of Testicular FSH Receptors and Its Application to Fetal Rat Testes(COMMUNICATION)(Endocrinology)

概要

論文の詳細を見る
The present study was undertaken to establish a micro-radioreceptor assay (micro-RRA) for FSH receptors, which enabled the reduction of the volume of reaction mixture to 40 μl from 200 μl in the standard RRA with the same concentrations of receptors and radioligands. In the micro-RRA system, the precision index (λ) was 0.17, and 50% displacement of binding of [^<13>I]iodo-rat FSH (NIAMDD-rat FSHI-4) to the FSH receptors was induced by 12ng of competitor/assay tube (competitor : NIH-FSH-P-2). In contrast in the standard system, λ was 0.10 and 50% displacement was induced by 55 ng of porcine FSH/ assay tube. The use of this micro-RRA on the study of FSH receptors in the rat testis during sexual maturation was equally satisfactory as the use of the standard RRA. There was no significant difference in the number of binding sites calculated from the Scatchard plot between the two assay system. The application of the micro-RRA for the determination of some properties of FSH receptors in the 19.5-day fetal rat testis showed that the number of binding sites and Kd were 1.4 frnol/ two testes and 0.27 nM, respectively. These results indicate that the micro-RRA system for FSH receptors established here is useful for the measurement of FSH receptors available only in minute quantities such as the fetal rat testis.
社団法人日本動物学会の論文
1985-04-15