Attempts to Improve Survival of Neurons Derived from Neonatal Rat Hypothalamus-Preoptic Area in Serum-free Media(Cell and Molecular Biology)

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In primary culture of cells derived from neonatal rat hypothalamus-preoptic area, a great decrease in the survival of neurons following an increase in the number of non-neuronal cells was observed in serum-supplemented medium. However, the addition of cytosine arabinoside, a mitotic inhibitor, to serum-supplemented medium significantly enhanced the survival of neurons. In order to diminish the proliferation of non-neuronal cells, attempts were made to maintain dissociated cells in serum-free medium. The maintenance of neurons in healthy condition was very difficult in serum-free medium from the very beginning of cultivation. Culture in serum-supplemented medium followed by culture in serum-free medium was much superior to the culture in serum-free medium for the entire course of cultivation with regard to survival. In the next experiment, the effects of glass surface and poly-lysine-coated surface were compared. Cultured cells in serum-free medium on glass surface were better in morphology compared with those on poly-lysine-coated surface. In the latter, strange contraction of basal cell sheets disturbed long-term cultivation. The best culture condition established in the present study, i.e., preculture in serum-supplemented medium and then transfer to serum-free medium on glass surface substrate, was employed for the study on the effects of hydrocortisone. Survival rate of neurons was improved in cultures with hydrocortisone at the concentration of 10^<-10> M.
社団法人日本動物学会の論文
1992-04-15

Attempts to Improve Survival of Neurons Derived from Neonatal Rat Hypothalamus-Preoptic Area in Serum-free Media(Cell and Molecular Biology)

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