Oxidized Low-Density Lipoprotein-Binding Specificity of the Asp-Hemolysin-Related Synthetic Peptides from Aspergillus fumigatus(Molecular and Cell Biology)

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概要

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• Oxidatively modified low-density lipoprotein (OxLDL) is present in atherosclerotic lesions and has been proposed to play an important role in atherogenesis. In the present study, in order to clarify the structure-binding activity relationship of Asp-hemolysin-related peptides to OxLDL, we investigated the interaction between Asp-hemolysin-related peptides consisting of 4 to 29 amino acid residues and OxLDL. The incubation of OxLDL with each Asp-hemolysin-related peptide resulted in the formation of an Asp-hemolysin/OxLDL complex. In particular, the tetrapeptide, YKDG (P-4), bound to OxLDL and inhibited the OxLDL-induced macrophage proliferation in a dose-dependent manner. Furthermore, we demonstrated that lysophosphatidylcholine (LysoPC) extracted from OxLDL inhibited the binding of P-21 to OxLDL in a dose-dependent manner and synthetic [^<14>C]LysoPC bound to P-21. We propose here that the YKDG region is one of the important sites for the binding of these peptides to OxLDL, and LysoPC as a typical lipid moiety of OxLDL is attributed to the binding of OxLDL to these peptides.

• 公益社団法人日本薬学会の論文
• 2006-11-01

著者

• Kumagai Takeshi

  Tohoku Pharmaceutical University

• Kumagai Takeshi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Kumagai Takeshi

  東北薬科大学 環境衛生学

• Kumagai T

  Tohoku Pharmaceutical Univ. Sendai Jpn

• NAGATA Kiyoshi

  Department of Pharmacology, School of Medicine, Keio University

• Nagata Kiyoshi

  Department Of Environmental And Health Science Tohoku Pharmaceutical University

• 蝦名 敬一

  東北薬大・環境衛生

• TSUTSUMI Hiromu

  Department of Environmental Health Science, Tohoku Pharmaceutical University

• OGAWA Norihiro

  Department of Environmental Health Science, Tohoku Pharmaceutical University

• NAITO Saori

  Department of Environmental Health Science, Tohoku Pharmaceutical University

• EBINA Keiichi

  Department of Environmental Health Science, Tohoku Pharmaceutical University

• YOKOTA Katsushi

  Department of Environmental Health Science, Tohoku Pharmaceutical University

• Naito Saori

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Ebina Keiichi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Ogawa Norihiro

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Kataoka Yumi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Yokota Katsushi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Nagata Kiyoshi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Kumagai Takeshi

  Department Of Electrical Engineering Nagaoka University Of Technology

• Tsutsumi Hiromu

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Kumagai Takeshi

  Department of Chemistry, Faculty of Science, Toho University

• NAGATA Kiyoshi

  Department of Drug Metabolism and Molecular Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University

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