A Novel Oxidized Low-Density Lipoprotein-Binding Protein,Asp-Hemolysin, Recognizes Lysophosphatidylcholine

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概要

• 論文の詳細を見る

• Oxidized low-density lipoprotein (Ox-LDL) plays an important role in the initiation and progression of atherosclerosis. Asp-hemolysin, a hemolytic toxin from Aspergillus fumigatus, is a specific, high affinity binding protein for Ox-LDL. We have previously shown that Ox-LDL strongly inhibits the hemolytic activity of Asp-hemolysin, and that the removal of lysophosphatidylcholine (lysoPC) from Ox-LDL abolished the inhibition. In the present study, to clarify the binding mechanism of Asp-hemolysin to Ox-LDL, we investigated the interaction between Asp-hemolysin and lysoPC as a typical lipid moiety of Ox-LDL. Based on western blot analysis, the binding of Asp-hemolysin to LDL, oxidized for different times, depended on the lysoPC content in each Ox-LDL. In addition, the inhibition of lysoPC production in Ox-LDL by phenylmethylsulfonyl fluoride (PMSF) pretreatment of LDL resulted in a marked decrease of Asp-hemolysin binding to PMSF-pretreated Ox-LDL. Furthermore, the binding analysis of Asp-hemolysin to lysoPC using ion-exchange chromatography revealed that Asp-hemolysin directly binds to lysoPC.

• 公益社団法人日本薬学会の論文
• 2002-06-01

著者

• Kumagai Takeshi

  Tohoku Pharmaceutical University

• Kumagai Takeshi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Kumagai Takeshi

  東北薬科大学 環境衛生学

• Kumagai T

  Tohoku Pharmaceutical Univ. Sendai Jpn

• 蝦名 敬一

  東北薬大・環境衛生

• YOKOTA Katsushi

  Department of Environmental Health Science, Tohoku Pharmaceutical University

• KUDO Yoichi

  Hygiene Research Laboratory, Sendai Hospital of East Japan Railway Company

• OOTANI Toshihiro

  The First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• KUMAGAI Takeshi

  The First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• FUKUCHI Yuji

  Japanese Red Cross Sendai Hospital

• EBINA Keiichi

  The First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• YOKOTA Katsushi

  The First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• Kudo Y

  Hygiene Research Laboratory Sendai Hospital Of East Japan Railway Company

• Ebina Keiichi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Kataoka Yumi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Ootani Toshihiro

  The First Department Of Hygienic Chemistry Tohoku Pharmaceutical University

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