Synthetic Peptide (P-21) Derived from Asp-Hemolysin Inhibits the Induction of Apoptosis on HUVECs by Lysophosphatidylcholine(Molecular and Cell Biology)

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概要

• 論文の詳細を見る

• Lysophosphatidylcholine (LPC), formed during low-density lipoprotein (LDL) oxidation and located within atherosclerotic plaques, regulates a variety of cellular functions, some of which could be construed to promote atherosclerotic lesion development, including vascular muscle cell proliferation, monocyte attraction, and endothelial cell apoptosis. We have previously reported that the synthetic peptide derived from Asp-hemolysin, named P-21, inhibits oxidized LDL (OxLDL)-induced macrophage proliferation through binding of P-21 to OxLDL. In this study, to clarify the interaction between P-21 and LPC as a typical lipid moiety of OxLDL, we examined the influence of P-21 on LPC-induced apoptosis in human umbilical vein endothelial cells (HUVECs). Based on flow cytometric analysis, using annexine V-fluorescein isothiocianate and propidium iodide as probes to assess apoptosis, LPC induced the apoptosis of HUVECs, and P-21 significantly inhibited this activity by 82.4%. Furthermore, dissociation-enhanced lanthanide fluorometric immunoassay indicated that LPC inhibited the binding of P-21 to OxLDL in a dose-dependent manner. A 50% inhibition dose was estimated to be 4.65μM of LPC. These results suggest that P-21 inhibits LPC-induced HUVEC apoptosis through binding of P-21 to LPC.

• 公益社団法人日本薬学会の論文
• 2006-05-01

著者

• Kumagai Takeshi

  Tohoku Pharmaceutical University

• Kumagai Takeshi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Kumagai Takeshi

  東北薬科大学 環境衛生学

• Kumagai T

  Tohoku Pharmaceutical Univ. Sendai Jpn

• YOKOTA Katsushi

  First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• 蝦名 敬一

  東北薬大・環境衛生

• TSUTSUMI Hiromu

  Department of Environmental Health Science, Tohoku Pharmaceutical University

• YOKOTA Katsushi

  Department of Environmental Health Science, Tohoku Pharmaceutical University

• TSUTSUMI Hiromu

  First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• KUMAGAI Takeshi

  First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• NAITOO Saori

  First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• EBINA Keiichi

  First Department of Hygienic Chemistry, Tohoku Pharmaceutical University

• Naito Saori

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Ebina Keiichi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Kataoka Yumi

  Department Of Environmental Health Science Tohoku Pharmaceutical University

• Yokota Katsushi

  First Department Of Hygienic Chemistry Tohoku College Of Pharmacy

• Tsutsumi Hiromu

  Department Of Environmental Health Science Tohoku Pharmaceutical University

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