Cryopreservation of Blue Honeysuckle in vitro-cultured Tissues Using Encapsulation-dehydration and Vitrification(平成17年度 第51回低温低物工学会研究報告)
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概要
- 論文の詳細を見る
Shoot apices of blue honeysuckle (Lonicera caerulea L. var. emphyllocalyx Nakai) in vitrcrcultures were successfully cryopreserved utilizing two methods of encapsulation-dehydration and vitrification. Encapsulation-dehydration, however, seems preferable to vitrification, since the highest survival rate of apices (93.3±3.3%) in encapsulation-dehydration was higher than that (50.0±5.8%) in vitrification. The highest survival was achieved when moisture content in beads was reduced to 23% by air-drying with silica gel for 4 hours in encapsulation-dehydration. In both cases, cold acclimation of cultures at 5℃ for 2 weeks was essential. However, in the present study it was shown that immersion of alginate-coated apices for 16 hours in loading solution supplemented with 0.5M glycerol and 0.1mM salicylic acid resulted in a high survival rate (93.3±6.7%) that can eliminate the cold acclimation process.
- 低温生物工学会の論文
- 2005-12-30
著者
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Kami Daisuke
Graduate School of Agriculture, Hokkaido University
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Oosawa Katsuji
Graduate School of Agriculture, Hokkaido University
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Oosawa Katsuji
Graduate School Of Agriculture Hokkaido University
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Kami Daisuke
Graduate School Of Agriculture Hokkaido University
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Suzuki Takashi
Department Of Biochemistry University Of Shizuoka School Of Pharmaceutical Sciences:core Research Fo
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KAMI Daisuke
Department of Horticulture and Landscape Architecture, Graduate School of Agriculture, Hokkaido Univ
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OOSAWA Katsuji
Department of Horticulture and Landscape Architecture, Graduate School of Agriculture, Hokkaido Univ
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Suzuki Takashi
Department Of Anesthesia Showa University Northern Yokohama Hospital
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Suzuki Takashi
Department Of Anatomic Pathology Tohoku University School Of Medicine
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