Position Alignment in Algebraic Reconstruction Method by Using Center of Gravity

概要

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The algebraic method was applied for transmission electron microscope (TEM) images taken from four directions. The reconstruction accuracy of this method was then evaluated for a 3-dimensional specimen structure. The reconstruction error was simulated by using various images which contain projection error, dark cuurent noise, and position error. A 10% projection error and 20-dB dark current noise in the projection image resulted in reconstruction errors of 15% and 13%, respectively. On the other hand, the position error influence was so extreme that 58% reconastruction error was induced by displacement of only ±4-pixels between the projection images. It was found that a position alignment of images within ±1 pixel was necessary in order to achieve proper reconstruction. A new position alignment method using the center of gravity of a projection image is applied for TEM images of uranyl acetate. Using this alignment method, a 3-dimensional structure was successfully reconstructed from four images.
社団法人応用物理学会の論文
1998-03-15