韓国における PCR によるツツガムシ個体からのツツガムシ病リケッチア DNA の検出
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概要
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In October 1995,field rodents and insectivores were captured at 5 localities in Chollanam-do and 3 localities in Choongchongnam-do, and a total of 81 field mice and shrews, including 64 Apodemus agrarius mice, were collected. The antibody positive rate of A. agrarius sera to Orientia tsutsugamushi was 52.4% in average. A total of 6,372 chigger mites were collected from the mice, and 10 species of 4 genera were identified. Leptotrombidium pallidum was predominant in Choong-chongnam-do, whereas L. scutellare was predominant in Chollanam-do. The chigger mites were individually dissected and internal contents were examined for O. tsutsugamushi infection applying IFA and PCR methods. Of 4,315 chigger mites screened by IFA, 115 positive and suspected cases were confirmed by consensus PCR. O. tsutsugamushi was found in 65 chiggers of 5 species, showing 1.51% infection rate in total. The infection rate of each 5 species was 1.64% in L. pallidum, 1.37% in L. scutellare, 1.77% in L. palpale, 1.60% in L. orientale and 1.41% in L. zetum. The detection of O. tsutsugamushi in L. orientale and L. zetum is the first finding in the world. The serotype of O. tsutsugamushi were also studied by analyzing the nucleotide sequences of the strain-specific region of 56 kDa protein, and all of the eight cases were of the F756-like type. This new sero-type was found from 4 species of Leptotrombidium, L. pallidum, L. scutellare, L. palpale and L. orientale.
- 日本衛生動物学会の論文
- 1997-09-15
著者
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LEE In
Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterina
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Lee In
Department Of Parasitology College Of Medicine Yonsei University
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REE Han
Department of Parasitology, College of Medicine, Yonsei University
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CHANG Woo
Department of Microbiology, College of Medicine, Hallym University
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KEE Sunho
Department of Microbiology, College of Medicine, Hallym University
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JEON Soung
Department of Parasitology, College of Medicine, Yonsei University
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Ree H‐i
Department Of Parasitology College Of Medicine Yonsei University
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Ree Han
Department Of Parasitology College Of Medicine Yonsei University
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Kee Sunho
Department Of Microbiology College Of Medicine Hallym University
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Jeon Soung
Department Of Parasitology College Of Medicine Yonsei University
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Chang Woo
Department Of Microbiology College Of Medicine Hallym University
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Chang Woo-hyun
Department Of Microbiology College Of Medicine Hallym University
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Lee In
Department Of Agronomy College Of Agriculture And Life Sciences Kyungpook National University
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Chang Woo
Department Of Internal Medicine Cardiology Sungkyunkwan University School Of Medicine
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Lee In
Department Of Aerospace Engineering
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Chang Woo
Department Of Horticulture National Chung Hsing University
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Ree Han-Il
Department of Parasitology, College of Medicine, Yonsei University
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