蚊の吸血量とミクロフィラリア摂取数の個体別測定法

概要

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We examined the new method for measuring the amount of blood meal in individual mosquitoes using a spectrophotometric technique and the number of microfilariae by membrane filtration method. Aedes togoi were fed on jird parasitized with Brugia malayi and frozen at -20℃ immediately. The midgut containing blood meal was homogenized in 2ml Drabkin's solution [1.0g NaHCO_3,0.1g K_2CO_3,0.05g KCN, 0.2g K_3Fe (CN)_6,in 1 liter distilled water], and incubated at room temperature (20-25℃) for 30min. The homogenate was filtered through Membrane filter (pore size=3μm). The filtrate was transferred to a microcuvette and the optical absorbance was read in a spectrophotometer at 540nm. The blood volume was calculated from the relationship between blood volume and optical absorbance calibrated previously. The homogenizer was washed with 4ml distilled water and the water was filtered through the same membrane to collect the suspected remainder of microfilariae. Then, the membrane was transferred on a glass slide and stained with 0.05% Azur II solution. The number of microfilariae was counted under a microscope. The contamination of Malpighian tubule caused the increase of optical absorbance equal to 0.4 μl blood. The homogenized sample was stable for 2hr and the suitable clearance of homogenizer was 0.2mm in this case.
日本衛生動物学会の論文
1980-12-15