蚊類のデングウイルス感受性に関する研究 : II. ウイルス実験宿主としての Toxorhynchites amboinensis (Doleschall) の継代飼育法の改良

概要

論文の詳細を見る
The purpose of this study was to examine methods of improving the mass rearing techniques of a non-biting mosquito Toxorhynchites amboinensis (Doleschall), which is an efficient laboratory host for dengue virus when the virus is injected intrathoracically. Experiments were directed at (1) the establishment of individual rearing techniques thus preventing larvae cannibalism; (2) the development of a suitable food for the larvae; and (3) the definition of the optimum physical conditions for larvae growth. 1. The larvae were reared individually in 3ml disposable trays containing 2ml of water or in 200ml plastic cups containing 50ml of water with Aedes albopictus (Skuse) larvae provided as food. The larval period at 27℃ was 18.1 days with a pupation rate of 89.1% in the disposable trays and 18.6 days and 94.1% in the plastic cups. In group rearing of 50 larvae in plastic trays (27×35×8cm) containing 3 liters of water, the larval period was 18.1 days and the pupation rate was 29.9%. It was shown that the individual method gives higher pupation rates and that the use of larger containers give slightly better results. 2. The larvae were reared using the following feed : Tetramine^[○!R], Vita-shrimp^[○!R], liver powder, dried water fleas, dried blood worms, and drone powder. The average larval periods were 47.7,45.5,56.0,53.7,43.7 and 52.0 days, respectively. The pupation rates were 70,70,50,80,40 and 10%, respectively. Larvae reared with a combination of a living diet of newly hatched brine shrimp Artemia salina (Linnaeus) for the young larval stage and the blood worm Limnodrilus hoffmeisteri Claparede for the third and fourth larval stages pupated after an average of 23.2 days at 25℃ with a pupation rate of 100%. These results were almost the same or slightly better than those obtained with group rearing by the conventional method providing Aedes albopictus larvae in which the larval period was 24.1 days and the pupation rate 90%. 3. The larvae were reared by providing a combined living diet at the temperature levels of 20,25,27 and 30℃. No larval growth was seen at 20℃, and the average preadult periods and pupation rates at the higher temperatures were 32.2,29.4 and 27.4 days, and 100,90 and 80%, respectively. At 30℃ the death rate of the blood worms was high enough to cause fouling of the water.
日本衛生動物学会の論文
1982-03-15

蚊類のデングウイルス感受性に関する研究 : II. ウイルス実験宿主としての Toxorhynchites amboinensis (Doleschall) の継代飼育法の改良

スポンサーリンク

概要

著者

関連論文

スポンサーリンク