LIGHT-INDUCED REDUCTION AND OXIDATION OF PLASTOCYANIN BY CHLOROPLAST PREPARATIONS
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概要
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In the light the intact chloroplasts of spinach reduce plastocyanin with a reaction rate comparable to that of the usual HILL reaction, while no reduction nor oxidation of the copper protein is induced by the chloroplasts in the dark. The dependency of the rate of photoreduction upon light intensity, pH and presence of various reagents was similar to that of the HILL reaction with the usual HILL oxidants. The photoreduction of plastocyanin was accelerated by the addition of complete phosphorylating system or ammonium sulfate to the chloroplasts. Digitonin-treated chloroplast was found to be inactive in photoreducing plastocyanin but highly active in photooxidizing reduced plastocyanin. The rate of photooxidation was saturated at about 5,000 lux, and showed a rather broad pH optimum around pH 8.0-8.5. The effects of various poisons on the reaction rate were studied. When the digitonin-treated chloroplasts were fractionated with ethanol into a chlorophyll-bearing particulate fraction and a soluble fraction, the former was active in catalyzing the photooxidation of reduced plastocyanin, but not in photooxidizing reduced cytochrome c. An appreciable photooxidation of reduced cytochrome c with the ethanol-precipitated fraction was obtained on addition of the soluble fraction, which was effectively replaced by plastocyanin. The properties of the reaction system responsible for photooxidation of plastocyanin and cytochrome c were compared, and a possible role of plastocyanin in the photooxidatory process of the chloroplasts was suggested.
- 日本植物生理学会の論文
著者
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Katoh Sakae
Department Of Biophysics And Biochemistry Faculty Of Scienee University Of Tokyo
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Takamiya Atusi
Department Of Biophysics And Biochemistry Faculty Of Scienee University Of Tokyo:(present)c.f.ketter
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Takamiya Atusi
Department Of Biochemistry Faculty Of Science University Of Tokyo
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Katoh Sakae
Department Of Biochemistry Faculty Of Science University Of Tokyo
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Katoh S.
Department of Biophysics and Biochemistry, Faculty of Scienee, University of Tokyo
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