Different Localization of two Ca^<2+> in Spinach Oxygen-Evolving Photosystem II Membranes. Evidence for Involvement of Only One Ca^<2+> in Oxygen Evolution
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概要
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Localization of the two Ca^<2+> bound to oxygen-evolving photosystem II (PSII) membranes from spinach was investigated by fractionating the membranes into the PSII reaction center core complexes and peripheral antenna Chl a/b-proteins after solubilization with n-heptylthioglucoside. The core complex fraction contained one Ca^<2+> per PSII, while another Ca^<2+> was found in the solubilized major light-harvesting Chl a/b-proteins (LHCII). LHCII isolated with Triton X-100 or dodecylmaltoside also contained Ca^<2+> in an amount corresponding to one per PSII. The Ca^<2+> bound to LHCII could not be removed by treatment with Chelex 100, which effectively sequestered extraneous Ca^<2+> bound to LHCII, or by preparation of LHCII in the presence of 40 mM citrate. Localization of the two Ca^<2+> in different functional domain of PSII membranes conclusively indicates that the number of the bound Ca^<2+> that can function in oxygen evolution is one per PSII. The results also suggest that one Ca^<2+> has a structural role in the peripheral antenna assembly.
- 日本植物生理学会の論文
著者
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Han Kab-cho
Department Of Biology Faculty Of Science University Of Tokyo
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Katoh Sakae
Department Of Biochemistry Faculty Of Science University Of Tokyo
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