Preparation and Characterization of P700-Enriched Photosystem-I Complexes from the Thermophilic Cyanobacterium, Synechococcus sp
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概要
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P700 was enriched relative to antenna pigments by treating the thylakoid membranes from thermophilic cyanobacterium (Synechococcus sp.) with diethylether. The total Chl α/P700 ratio of the membranes was 147 but decreased to 13 after the treatment with ether which had been 70% saturated with water. Vitamin K_1 and carotenoids were completely removed by this treatment. The low chlorophyll a to P700 ratio was retained in photosystem I reaction-center complexes purified from the ether-extracted membranes with Triton X-100. The midpoint potential of P700 was considerably lowered by the ether treatment of the thylakoid membranes but was partially reversed by the further treatment of the extracted membranes with Triton X-100. Photo-oxidation of P700 in the purified complexes was extremely slow under continuous illumination, indicating that there is no significant leakage of electrons from the primary electron acceptor to other bound acceptors or O_2. The photooxidation of P700 was appreciably accerelated on addition of vitamin K_3 (or K_1).
- 日本植物生理学会の論文
著者
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Katoh Sakae
Department Of Biology Faculty Of Science Toho University
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Katoh Sakae
Department Of Pure And Applied Sciences. College Of Arts And Sciences University Of Tokyo
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lkegami Isamu
Laboratory of Chemistry, Faculty of Pharmaceutica! Sciences, Teikyo University
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Lkegami Isamu
Laboratory Of Chemistry Faculty Of Pharmaceutica! Sciences Teikyo University
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Katoh Sakae
Department Of Biochemistry Faculty Of Science University Of Tokyo
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