Preparation and Characterization of Heat-Stable and Very Active Oxygen-Evolving Photosystem II Particles from the Thermophilic Cyanobacterium, Synechococcus elongatus
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概要
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Very active and heat-stable oxygen-evolving photosystem II particles were isolated from the thermophilic cyanobacterium Synechococcus elongatus by treatment of thylakoid membranes with a non-ionic detergent, sucrose monolaurate (SML). The particles were analyzed in a com-parison with photosystem II particles prepared with β-octylglucoside (OG). The two preparations had similar polypeptide compositions, which were caracterized by high levels of polypeptides from phycobilisomes. The ratio of chlorophyll a to Q_A was 45 and there were four Mn atoms and one tightly bound Ca^<2+> ion per Q_A in the particles prepared with SML. The preparations were thermophilic, showing substantial rates of oxygen evolution at temperatures up to 60℃. The maximum rates attained at 45℃ were as high as 6.0 mmoles O_2 mg^<-1> Chl h^<-1>. PS II particles prepared with OG were similarly thermostable but were less active in oxygen evolution at all temperatures examined. Kinetic analysis of flash-induced absorption transients revealed that about 22% and 28% of photosystem II reaction centers were not associated with the functional Q_B site in the SML- and OG-particles, respectively. When corrected for the inactive reaction centers, the maximum rates of oxygen evolution by SML- and OG-particles were 7.7 and 7.0 mmoles O_2 mg^<-1> Chl h^<-1>, which correspond to half times of 1.9 and 2.1 ms for the first-order electron transfer, respectively. Comparison of these half times with those of the S-state transition and the release of oxygen indicates that the overall photosystem II electron transport is limited by the reduction of added electron acceptors and not by release of oxygen.
- 日本植物生理学会の論文
著者
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Satoh Kazuhiko
Department of Life Science, University of Hyogo, 3-2-1 Kohto, Kamigohri, Ako-gun, Hyogo 678-1297, Ja
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Katoh Sakae
Department of Pure and Applied Sciences. College of General Education. University of Tokyo
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Satoh Kazuhiko
Department Of Biology Faculty Of Science Toho University
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Satoh Kazuhiko
Department Of Biology Faculty Of Science University Of Tokyo
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Ichimura Toshiaki
Department Of Biology Faculty Of Science University Of Tokyo
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Katoh Sakae
Department Of Biology Faculty Of Science Toho University
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Miyairi Sachio
National Chemical Laboratory for Industry
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Miyairi S
National Chemical Lab. Industry Ibaraki‐ken
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Katoh Sakae
Department Of Biochemistry Faculty Of Science University Of Tokyo
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