Parallel time courses of electrochromic shifts of carotenoids and cytochrome f photooxidation in intact Bryopsis chloroplasts

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Light-induced absorbance changes at 560 nm, and electrochromic shifts in absorption of carotenoids responding to membrane potential across the thylakoid membrane, were studied, comparing them with the kinetics of cytochrome f photooxidation, in the dark-adapted intact chloroplasts of the green alga Bryopsis maxima. The 560 nm changes showed transient variations, characterized by a sharp initial peak followed by a second, lower peak, within a few seconds of illumination. The. time course of the 560 nm changes is parallel to that of light-induced transient changes of cytochrome f. Inhibitors and redox substances which selectively influenced different transient phases of the cytochrome f induction also specifically affected corresponding transient phases of the 560 nm changes. These results indicate that the two induction phenomena are closely related to each other and that the induction of the 560 nm change is due to light-dependent changes in electron transfer on the reducing side of photosystem I. A possible mechanism for the electric field formation by electron transfer associated with photosystem I will be discussed.
日本植物生理学会の論文