Ferricyanide-induced absorbance change of carotenoid in chromatophores of Rhodopseudomonas spheroides correlated to the oxidation of bacteriochlorophyll 885
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概要
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Addition of high concentrations (e.g., 1-100m_M) of ferricyanide to a chromatophore suspension of Rhodopseudomonas spheroides caused a change in the absorption spectrum of carotenoid (spheroidene), which was completely reversed by adding reducing reag cllts such as ferrocyanide and ascorbate. The spectral change is represented by a shift in the absorption spectrum of carotenoid by 2 to 2.5nm towards the longer wavelength side. The presence of piericidin A, o-phenanthroline or Cl-CCP in the reaction mixture did not affect the ferricyanide-induced absorbance change. Triton X-100 markedly suppressed the magnitude of the change. The addition of ferricyanide also caused simultaneous absorbance changes with maxima at 590 and 885nm. These are ascribed to oxidation of the (bulk) bacteriochlorophyll, BChl 885. There was no absorption change at other peaks of bacteriochlorophyll in the infrared region (i.e., 800 and 855nm). Therefore, the ferricyanide-induced absorbance change of carotenoid did not represent an oxidation-reduction reaction of carotenoid but was intimately correlated with oxidation of BChl 885 in the chromatophores, as judged from similarities observed with respect to the time course patterns, midpoint potential (545-555mv) in the ferri-ferrocyanide reaction system, as well as behavior towards various reagents and inhibitors added A similar change of carotenoid (i.e., 2-2.5nm shift of absorption spectrum) was caused by addition of MgCl_2 to the chromatophores, but this did not induce any change in the absorption spectrum of bacteriochlorophyll. The nature of the spectral change of carotenoid in chromatophores is discussed.
- 日本植物生理学会の論文
著者
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Takamiya Atusi
Department Of Biochemistry Faculty Of Science University Of Tokyo
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Okada Mitsumasa
Department Of Biochemistry Faculty Of Science University Of Tokyo
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