Purification and Further Characterization of Pyrenoid Proteins and Ribulose-1,5-bisphosphate Carboxylase-Oxygenase from the Green Alga Bryopsis maxima
スポンサーリンク
概要
- 論文の詳細を見る
Pyrenoid proteins and ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) in the green alga Bryopsis maxima were purified to high degrees and their peptide compositions were studied by SDS-polyacrylamide gel electrophoresis. RuBisCO had a large subunit of 50 kDa and a small one of 16 kDa. The apparent molecular weight of the purified RuBisCO was estimated as 460 kDa by gel filtration. Pyrenoid proteins had two major polypeptides: 52 kDa and 17 kDa. The peptide map of the 52 kDa pyrenoid polypeptide coincided well with that of the large subunit of RuBisCO, strongly suggesting that the major component of the pyrenoid of this alga was RuBisCO. We attempted to survey the distribution of RuBisCO in the chloroplasts. The results suggested that much of the RuBisCO of Bryopsis maxima was localized in the pyrenoid. The pyrenoid also contained more than 10 minor polypeptides not found in the RuBisCO fraction. The minor polypeptides comprised about 15% of the total pyrenoid protein and differed from the polypeptides of the thylakoid membranes and from those found in the starch grains surrounding the pyrenoid.
- 日本植物生理学会の論文
著者
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Satoh Hikaru
Institute Of Genetic Resources Faculty Of Agriculture Kyushu-university
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Okada Mitsumasa
Department Of Biomolecular Science Toho University
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Okada Mitsumasa
Department Of Biomolecular Science Faculty Of Science Toho University
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Satoh H
Institute Of Genetic Resources Faculty Of Agriculture Kyushu-university
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Nakayama K
Toho Univ. Chiba Jpn
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Nakayama Katsumi
Department Of Biomolecular Science Toho University
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Nakayama Katsumi
Department Of Biology Faculty Of Science Toho University
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Miyaji Kazuyuki
Department Of Biology Faculty Of Science Toho University
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Satoh Hiroyuki
Department Of Biomolecular Science Toho University
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Okada Mitsumasa
Department Of Biochemistry Faculty Of Science University Of Tokyo
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Satoh Hiroyuki
Department Of Biology Faculty Of Science Toho University
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