Biosynthesis of Abscisic Acid in the Fungus Cercospora cruenta : Stimulation of Biosynthesis by Water Stress and Isolation of a Transgenic Mutant with Reduced Biosynthetic Capacity : ENVIRONMENTAL AND STRESS RESPONSES
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概要
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The rate of synthesis of abscisic acid (ABA) by the phytopathogenic fungus Cercospora cruenta was found to be increased 2.6-fold by water stress (0.5 M mannitol, 48 h). To characterize the oxidative steps in the synthesis of ABA by C. cruenta, a mutant strain deficient in the synthesis of ABA was isolated after transformation with a foreign gene. This strain was further characterized by an immunoassay using a monoclonal antibody specific for 1',4'-dihydroxy-γ-ionylideneacetate. One transformant that seemed likly to be deficient in the synthesis of ABA was isolated. Integration of the foreign gene into the chromosomal DNA of the transformant was confirmed by Southern hybridization.
- 日本植物生理学会の論文
著者
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KITAGAWA Yoshichika
Biotech. Inst., Akita Pref. Univ.
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Yamamoto Hiroshi
Department Of Energy Engineering And Science Nagoya University
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Oritani Takayuki
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Oritani Takayuki
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University
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Kitagawa Yoshichika
Biotechnology Institute Akita Prefectural College Of Agriculture
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Kitagawa Yoshichika
Biotech. Inst. Akita Pref. Coll. Agr.
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Yamamoto Hiroshi
Departmemt Of Chemical Engineering University Of Tokyo
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Yamamoto Hiroshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku University:(present)nitto C
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