Effect of cycloheximide and cordycepin on auxin-induced elongation and β-glucan degradation of noncellulosic polysaccharides of Avena coleoptile cell wall

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The effect of cycloheximide (10^<-5> _M) and cordycepin (10^<-4> _M) used as protein and RNA synthesis inhibitors, respectively, on auxin action in noncellulosic β-glucan degradation of Avena coleoptile cell wall was investigated. Both depressed auxin-induced β-glucan degradation of the cell wall as well as auxin-induced elongation and cell wall loosening, suggesting that the process of β-glucan degradation of the cell wall is closely associated with cell wall loosening and that auxin enhances the activity of an enzyme for β-glucan degradation through de novo synthesis of RNA and protein but not through activation of the enzyme in situ. Kinetic study with the inhibitors showed that RNA metabolism involved in β-glucan degradation was stimulated by auxin treatment of only 15 min while a longer lag phase (about 1 hr) existed for the synthesis of the enzyme.
日本植物生理学会の論文