Effect of Xyloglucan Nonasaccharide on Cell Elongation Induced by 2,4-Dichlorophenoxyacetic Acid and Indole-3-acetic Acid

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Xyloglucan nonasaccharide (XG9) is recognized as an inhibitor of 2,4-D-induced long-term growth of segments of pea stems. In the presence of 10^<-5> M 2,4-D, inhibition by 10^<-9> M XG9 of elongation of third internode segments of pea seedlings was detected within 2 h after the start of incubation, in some experiments. Analysis by double-reciprocal (Lineweaver-Burk) plots of elongation in the presence of various concentrations of 2,4-D, with or without XG9, gave parallel lines, indicating that XG9 inhibited 2,4-D-induced elongation in an uncompetitive manner. XG9 did not influence the 2,4-D-induced cell wall loosening. Thus, XG9 does not fulfill the proposed definition of an "antiauxin". XG9 at 10^<-11> to 10^<-6> M did not influence IAA-induced elongation of segments from pea third internodes, azuki bean epicotyls, cucumber hypocotyls, or oat coleoptiles. Inhibition of IAA-induced elongation by XG9 was not observed even when the segments from pea or azuki bean were abraded. Furthermore, fucosyl-lactose at 10^<-11> to 10^<-4> M did not affect the IAA-induced elongation of segments of pea internodes or of azuki bean epicotyls. XG9 may be incapable of inhibiting the IAA-induced cell elongation (especially in oat) or, alternatively, the endogenous levels of XG9 may be so high that exogenously applied XG9 has no inhibitory effect on IAA-induced elongation.
日本植物生理学会の論文