Amino Acid-Sugar Linkages in Rice Coleoptile Cell Walls

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The nature of amino acid-sugar linkages in cell walls was investigated in a monocotyledonous tissue, rice coleoptiles. The molar ratios of aspartic acid, threonine, and serine in cell walls were decreased by hydrazinolysis in coleoptiles grown both on and under water. The molar ratios of threonine and serine were decreased also by a NaOH-NaBH_4 treatment, while the alanine content was increased, and α-aminobutyric acid was not formed. The cell walls were treated with NaOH in the presence of NaB^3H_4, hydrolyzed, then divided into amino acid and sugar fractions. Two distinct radioactive peaks were detected in the thin-layer chromatography of the amino acid fractions. One was identified as alanine derived from glycosylated serine; the other was confirmed to be an oxidation product of glucosaminitol. There was just one 3H-labeled product in the sugar fractions, galactitol. These results suggest the presence of serine-O-galactose and asparagine-N-N-acetyl glucosamine linkages in rice coleoptile cell walls. The existence of glucosamine linked to amino acids was further supported by the incorporation of ^<14>C-glucosamine into cell walls. These linkages were also detected in the cell walls of a dicotyledonous tissue, Vicia epicotyls.
日本植物生理学会の論文