Natural Occurrence of Indoleacetamide and Amidohydrolase Activity in Etiolated Aseptically-Grown Squash Seedlings : PROTEINS, ENZYMES AND METABOLISM : NATURAL PRODUCTS
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概要
- 論文の詳細を見る
Etiolated seedling tissues of aseptically grown squash (Cucurbita maxima Duch) contain in-dole-3-acetamide (IAM) as a natural endogenous compound, conclusively identified by gas chromatography-mass spectrometry (GC-MS). Roots of aseptically raised seedlings also contain amide hydrolysing activity, which converts IAM to IAA, indoleacetonitrile (IAN) to IAM and IAA, and 1-naphthaleneacetamide to 1-naphthaleneacetic acid. This activity was enriched 48-fold by fractional precipitation with ammonium sulphate, Sephadex gel filtration and anion exchange chromatography. Being hydrolytic, it works equally well in air and in vacuo, without added cofactors. The partially purified enzyme works optimally between pH 7 and 7.5, and a K_m value of 80 μM was calculated with IAM as the substrate. The product of this reaction was definitively identified as IAA by GC-MS. The temperature optimum of this amidohydrolase lies around 45℃, and it is stable to freezing. A comparison of its properties with the amidohydrolase of Agrobacterium or crown gall tissue, shows it to be different. In view of the natural occurrence of both IAM and the amidohydrolase, it is suggested that the IAM pathway of IAA biogenesis is feasible in etiolated squash seedlings.
- 日本植物生理学会の論文
著者
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Kuraishi Susumu
Department of Environmental Studies; Faculty of Integrated Arts and Sciences, Hiroshima University
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Sakurai Naoki
Department Of Environmental Studies Faculty Of Integrated Arts And Sciences Hiroshima University
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Sakurai Naoki
Department Of Biology Faculty Of Science Osaka City University:(present)department Of Environmental
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Kannan-gara Gemini
Department Of Physiology Carisberg Research Centre
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RAJAGOPAL Ranganatha
Instutute for Plant Biology, Royal Vet. & Agri, University
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TSURUSAKI Ken-ichi
Department of Environmental Studies, Hiroshima University
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Tsurusaki K
Fukuyama Univ. Fukuyama Jpn
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Rajagopal Ranganatha
Instutute For Plant Biology Royal Vet. & Agri University
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Kuraishi Susumu
Department Of Botany University Of Iowa:(present)biological Institute College Of General Education U
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Kuraishi Susumu
Department Of Environmental Studies Hiroshima University
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Kuraishi Susumu
Department Of Biology College Of Education University Of Tokyo:(present)department Of Enviromental S
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Sakurai Naoki
Department Of Environmental Studies Hiroshima University
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Sakurai Naoki
Department Of Biology
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