A Simplified Method for the Quantitative Determination of Indoleacetic Acid by High Performance Liquid Chromatography with a Fluorometric Detector

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概要

• 論文の詳細を見る

• Quantitative analysis of endogenous IAA contents of radish plant extracts by high performance liquid chromatography with a fluorometric detector was refined by using a C-18 SEP-PAK cartridge for the purification procedure and indolepropionic acid as the internal standard. The C-18 SEP-PAK cartridge efficiently purified the IAA from the acidic ether-soluble fraction of the plant extracts when the pH of the fraction injected into the cartridge was kept under 3.5. Both of the recovery rates for IAA and indolepropionic acid were over 90% for the extraction and purification procedure. The endogenous IAA content in green radish seedlings, corrected by internal standard methods with indolepropionic acid, was 20.2ng/g fr wt. This was in good agreement with the value corrected with radiolabeled ^<14>C-IAA, 20.8 ng/g fr wt.

• 日本植物生理学会の論文

著者

• Sakurai N

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Sakurai Naoki

  Department Of Biology Faculty Of Science Osaka City University:(present)department Of Environmental

• Kuraishi Susumu

  Department Of Botany University Of Iowa:(present)biological Institute College Of General Education U

• Akiyama Masaya

  Department of Environmental Studies, Faculty of Integrated Arts and Sciences, Hiroshima University

• Kuraishi Susumu

  Department Of Biology College Of Education University Of Tokyo:(present)department Of Enviromental S

• Sakurai Naoki

  Department Of Biology

• Akiyama Masaya

  Department Of Environmental Studies Faculty Of Integrated Arts And Sciences Hiroshima University

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