Simultaneous Determination of Esterase and Peptidase Activities of Elastase

概要

論文の詳細を見る
1. Synthetic substrates conventionally used can give either the esterase or peptidase activity of elastase separately, but simultaneous determination of both activities using one substrate has not previously been attempted. 2. In this work, the esterase and peptidase activities of elastase were determined simultaneously. Esterase activity can be expressed in terms of formation of Ala_3 and peptidase activity can be expressed in terms of formation of Ala_2 with Ala_3OMe as the substrate : Ala_3OMe is split into Ala_3+MeOH by the esterase activity of elastase and into Ala_2+Ala-OMe by the peptidase activity. 3. The present method differentiates the esterase or peptidase activities of other enzymes from those of elastase. Ala_3OMe is split to Ala_3,and then to Ala_2+Ala in a stepwise manner by other proteases such as collagenase and pronase E. 4. Trypsin and chymotrypsin enhanced both the peptidase activity and esterase activity of elastase in parallel. 5. The substrate, suc Ala_3NA has been used conventionally to determine elastase activity, and the results with suc Ala_3NA and Ala_3OMe showed a good correlation.
社団法人日本薬学会の論文
1980-01-25