ヨウ素化合物の研究(第7報)酸素フラスコ燃焼法及び固相濃縮法を利用した高速液体クロマトグラフィーによるThyroglobulin中の結合ヨウ素の定量

概要

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A sensitive method was investigated for the determination of binding iodine in thyroglobulin (TG). The method was based on the solid-phase preconcentration of iodide (I^-) in the absorbing solution prepared by the oxygen flask combustion of TG. The optimum procedures were discussed for the preconcentration. The absorbing solution was passed through a cation-exchange column (AG-50), followed by an anion-exchange column (Bond Elut PSA). Iodide concentrated on the Bond Elut PSA was eluted with ammonia water and treated with the AG-50. An aliquot of the concentrated sample was subjected to reversed-phase HPLC with a UV detector for the determination of I^-. The method was applied successfully to the determination of binding iodine in trace amounts of TG.
社団法人日本薬学会の論文
1994-08-25