プレカラム誘導体化・高速液体クロマトグラフィーによる製剤中のタウリン, L-グルタミン, ビタミンU, L-アスパラギン酸の定量

概要

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A pre-column derivatization method for the high-performance liquid chromatographic determination of taurine (1), L-glutamine (2), vitamin U (3) and L-aspartic acid (4) in pharmaceuticals has been developed. The optimum requirements for the derivatization conditions and the stability of resulting derivatives were discussed. The compounds were converted into DNT derivatives through the amino group by reaction with sodium 2,6-dinitro-4-trifluoromethylbenzenesulfonate (DNTS) in 50% sodium borate at 60℃ for 30 min (1), at 60℃ for 90 min (2), at 60℃ for 80 min (3) and at 80℃ for 90 min (4). After the reaction mixtures were acidified with dil. HCl, the derivatives were separated on a Cosmosil 3C_<18> (4.6mm i.d.×50mm) column using 1% acetic acid-methanol (13 : 7) containing 2mM sodium 1-heptanesulfonate as mobile phase with a ultra violet detector at 280nm. The precisions of the analytical values expressed as the coefficient of variation were below 2.0%. The recoveries of 1-4added to various commercial samples were in the range of 97.8-100.6%.
公益社団法人日本薬学会の論文
1994-09-25