高速液体クロマトグラフィーによるセンブリ配合育毛剤中のスウェルチアマリンの定量

概要

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A method for the determination of swertiamarin (SA) in hair tonics containing Swertia japonica extract by use of high performance liquid chromatography (HPLC) was developed. A sample of hair tonic was evaporated twice with ethanol under reduced pressure and dissolved by adding water. The test solution was prepared by passing through a Sep-Pak C_<18> cartridge, washing with water and eluting with a mixture of acetonitrile-water (2 : 8). SA in the eluate was determined by HPLC on an octadecylsilylated silica gel column (TSKgel-ODS 80T_M) by using a mobile phase of acetonitrile-water (12 : 88) and 235 nm for the detection. The present method was applicable to the determination of SA in commercial hair tonics without interference of other cosmetic ingredients.
公益社団法人日本薬学会の論文
1989-10-31