Is K-ras Point Mutation an Early Event in Lung Tumorigenesis of Mice?

概要

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To investigate if K-ras point mutations could occur at an early stage of urethane-induced lung tumorigenesis in mice, we applied a sensitive mutation specific PCR assay technique (MSPA) to DNA from lung at the early stage of this condition. At 7 or 14 d after urethane injection (750 mg/kg, i. p.), pulmonary DNA was isolated and the existence of activated K-ras gene with 61st codon A→T mutation was checked according to the MSPA. This technique can detect mutant alleles in the presence of a very large excess of normal ras alleles ; however, the K-ras point mutation could not be detected by the present MSPA. These results suggest that the K-ras point mutations did not occur at the stage of lung tumorigenesis in mice or that the number of mutant alleles was below the detectable range of the technique.
社団法人日本薬学会の論文
1996-04-30