Further Metabolism of 2-Formylaminofluorene, a Metabolite of 2-Aminofluorene, in Rats

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The further in vivo metabolism of 2-formylaminofluorene (FAF), which is a metabolite of 2-aminofluorene, was examined in rats. The 48 h urine collected following oral administration of FAF to rats was treated with β-glucuronidase/arylsulfatase and extracted with benzene. When the benzene extract was subjected to HPLC, ten peaks due to FAF and its metabolites were detected. Among these metabolites, major metabolites 1,2,4 and 5 were isolated and identified unequivocally as 7-hydroxy-2-formylaminofluorene, 7-hydroxy-2-acetylaminofluorene, 5-hydroxy-2-formylaminofluorene and 5-hydroxy-2-acetylaminofluorene, respectively, by comparing their MS and UV spectra, and HPLC and TLC behavior with that of authentic specimens. Minor metabolites, 7-hydroxy-2-aminofluorene, 5-hydroxy-2-aminofluorene, 2-acetylaminofluorene and 2-aminofluorene together with the parent compound were detected by HPLC. These metabolites of FAF were also detected in the feces of rats given FAF.
1996-02-15