Real Time Measurement of Nitric Oxide Released from Cultured Endothelial Cells
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概要
- 論文の詳細を見る
Direct detection of nitric oxide (NO) is essential for understanding the precise mechanism of its production from endothelial cells. Previously, we developed an NO detection system based on the chemiluminescence reaction between NO and luminol-H_2O_2. Here, we have applied this system to cultured endothelial cells for the direct and on-time measurement of NO. The perfusate from cultured endothelial cells was continuously mixed with luminol-H_2O_2. N^G-monomethyl-L-arginine (L-NMMA) (10^<-4>M) decreased the chemiluminescence signal of NO, suggesting the existence of basal NO release. Bradykinin (10^<-8>-10^<-6>M) increased the NO signal (10^<-6>M;5.1±0.4 fmol/min, corresponding to 1.7 pM in the perfusate), and this was inhibited by 10^<-4>M L-NMMA (1.8±0.3 fmol/min). These results corresponded to the changes in cGMP levels in RFL-6 cells, which provide an No bioassay system. We conclude that the luminol-H_2O_2 system is useful for the direct and continuous measurement of NO from coltured endothelial cells.
- 公益社団法人日本薬学会の論文
- 1998-12-15
著者
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NAGAO Taku
Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, The Universit
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Nagao Taku
Laboratory Of Pharmacology & Toxicology Graduate School Of Pharmaceutical Sciences The Universit
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HIROBE Masaaki
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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Nagao T
Faculty Of Pharmaceutical Sciences Fukuoka University
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KURUMATANI Hajimu
Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, The Universit
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KIKUCHI Kazuya
Laboratory of Bioorganic and Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, The Un
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NAGANO Tetsuo
Laboratory of Bioorganic and Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, The Un
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HIROBE Masaaki
Laboratory of Bioorganic and Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, The Un
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YAMAZAKI Jun
Laboratory of Pharmacology and Toxicology, Graduate School of Pharmaceutical Sciences, The Universit
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Hirobe M
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Kikuchi K
Graduate School Of Pharmaceutical Sciences The University Of Tokyo:presto Jst Corporation
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Kurumatani Hajimu
Laboratory Of Pharmacology And Toxicology Graduate School Of Pharmaceutical Sciences The University
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Yamazaki J
Laboratory Of Pharmacology And Toxicology Graduate School Of Pharmaceutical Sciences The University
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