Cross-Linking of Collagen by Singlet Oxygen Generated with UV-A
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概要
- 論文の詳細を見る
Singlet oxygen (^1O_2), a highly reactive and toxic intermediate, may play a role in photo-induced aging. We examined singlet oxygen generation from hematoporphyrin (HP) with UV-A, by monitoring the emission at 1268nm corresponding to ^1O_2→^3O_2. Singlet oxygen was formed HP-dose-dependently in this system. We then investigated the reaction of singlet oxygen generated by UV-A irradiation with collagen, which is related to skin elasticity and softness. Collagen from skin was rapidly and dose-dependently cross-linked by singlet oxygen. The reaction was inhibited by NaN_3,a selective quencher of singlet oxygen. In contrast, SOD (superoxide dismutase) and mannitol had no effect. These results suggested that cross-linking of collagen was caused by UV-A-generated singlet oxygen, not by any other reactive oxygen species. Compared with another multisubunit protein, alcohol dehydrogenase, collagen was cross-linked much more efficiently. Further, the finding that semicarbazide inhibited cross-linking of collagen showed that cross-links were formed between photooxidized histidyl residues and amino groups. Singlet oxygen generated by UV-A irradiation may contribute to cross-linkin of collagen in the process of skin photoagaing.
- 公益社団法人日本薬学会の論文
- 1997-08-15
著者
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NAGANO Tetsuo
Faculty of Pharmaceutical Science, University of Tokyo
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HIROBE Masaaki
Graduate School of Pharmaceutical Sciences, The University of Tokyo
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HIROBE Masaaki
Faculty of Pharmacuetical Sciences, University of Tokyo
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荒金 久美
Research Laboratory, KOSE Corporation
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荒金 久美
Research Laboratory Kose Corporation
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Hirobe M
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
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Hirobe Masaaki
Faculty Of Pharmaceutical Science University Of Tokyo
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Nagano T
Lab. Of Chemistry And Biology Graduate School Of Pharmaceutical Sciences The Univ. Of Tokyo
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Nagano Tetsuo
Faculty Of Pharmaceutical Science University Of Tokyo
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RYU Akemi
Research Laboratory, KOSE Corporation
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NARU Eiji
Research Laboratory, KOSE Corporation
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ARAKANE Kumi
Research Laboratory, KOSE Corporation
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MASUNAGA Takuji
Research Laboratory, KOSE Corporation
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SHINMOTO Koichi
Research Laboratory, KOSE Corporation
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MASHIKO Shinrou
Communications Research Laboratory
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Naru Eiji
Research Laboratory Kose Corporation
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Shinmoto K
Research Laboratory Kose Corporation
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Mashiko S
Communications Research Laboratory
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Masunaga T
Research Laboratory Kose Corporation
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Ryu Akemi
Research Laboratories, KOSÉ Corporation
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Arakane Kumi
Research Laboratories, KOSÉ Corporation
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