EVALUATION OF METHODS FOR DURATION OF PRESERVATION OF RNA QUALITY IN RAT LIVER USED FOR TRANSCRIPTOME ANALYSIS
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概要
- 論文の詳細を見る
In The Toxicogenomics Project, about 150 chemicals are administered to rats, and gene expression in the liver analyzed by Affymetrix GeneChip and stored in the database. As the quality of RNA greatly influences the accuracy of gene expression data, conditions of the storage of the sample are very important. Recently, an RNA stabilization solution, RNAlater^[○!R], has become commercially available. In this study, the new storage method was compared with the traditional storage method (stored in freezer or liquid nitrogen) under various conditions by looking at the degradation of RNA assessed by its total yield, OD260/280 ratio, 28S/18S ratio, and quantity of β-actin. It was confirmed that RNAlater^[○!R] preserved the liver tissue sample by maintaining the quality of RNA for one year (in liquid N_2 or -80℃), for 3 days (4℃), or for 2 hr (room temperature) without degradation of RNA. Quality of RNA samples dissolved in buffer RLT and stored at -20℃ tended to decrease, but samples stored at -80℃ were almost equivalent to those stored in liquid nitrogen. In conclusion, we recommend the following procedure for preservation of liver tissue for extraction of RNA: 1) tissues removed should be put into chilled RNAlater^[○!R] as soon as possible; 2) samples in RNAlater^[○!R] must be stored overnight or longer at 4℃ and can be left for as long as 2 weeks without freezing; 3) samples in RNAlater^[○!R] can be stored for at least one year under less than -20℃ and 4) samples dissolved in buffer RLT can be preserved at least for one year under -80℃.
- 日本トキシコロジー学会の論文
- 2006-12-15
著者
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NAGAO Taku
National Institute of Health Sciences
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Nagao Taku
Food Safety Commission Of Japan
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Nagao Taku
Laboratory Of Pharmacology & Toxicology Graduate School Of Pharmaceutical Sciences The Universit
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Nagao Taku
Laboratory Of Pharmacology And Toxicology Graduate School Of Pharmaceutical Sciences The University
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Ono Atsushi
Toxicogenomics Project National Institute Of Biomedical Innovation:national Institute Of Health Scie
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Miwa Satoko
Exploratory Toxicology & Dmpk Research Laboratories Tanabe Seiyaku Co. Ltd.
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KASAHARA Toshihiko
Toxicogenomics Project, National Institute of Biomedical Innovation
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MIYAGISHIMA Toshikazu
Toxicogenomics Project, National Institute of Biomedical Innovation
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URUSHIDANI Tetsuro
Toxicogenomics Project, National Institute of Biomedical Innovation
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Urushidani Tetsuro
Toxicogenomics Project National Institute Of Biomedical Innovation:department Of Pathophysiology Dos
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Urushidani Tetsuro
Laboratory Of Pharmacology & Toxicology Graduate School Of Pharmaceutical Sciences The Universit
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Miyagishima Toshikazu
Toxicogenomics Project National Institute Of Biomedical Innovation
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Nitta Hiroyuki
Toxicogenomics Project National Institute Of Biomedical Innovation
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Kasahara Toshihiko
Toxicogenomics Project National Institute Of Biomedical Innovation
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MIYAZAKI Toshiko
Toxicogenomics Project, National Institute of Biomedical Innovation
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Nagao Taku
National Institute Of Health Sci.
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Ono Atsushi
Toxicogenomics Project National Institute Of Biomedical Innovation:national Institute Of Health Scie
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Urushidani Tetsuro
Toxicogenomics Project National Institute Of Biomedical Innovation
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Ono Atsushi
Toxicogenomics Informatics Project, National Institute of Biomedical Innovation
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