Prevention of Co-elution of Steroid Sulfates with Serum Proteins from Pre-column in Column-switching HPLC System
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概要
- 論文の詳細を見る
A method to prevent co-elution of steroid sulfates with proteins in serum from the pre-column in column-switching HPLC was developed. The pre-column, a polymer-coated mixed function column, was used for ionpair chromatography with 5 mM tetra-n-butylammonium (TBA) ion. As steroid sulfates, estriol 3-sulfate, dehydroepiandrosterone 3-sulfate and pregnenolone 3-sulfate were used. Human serum (25 μl) was diluted with mobile phases including 5,100 and 500 mM TBA ion, and then injected directly into the pre-column. The peak areas of the steroid sulfates in serum samples were compared with those of the steroid standards without serum. When 25 μl of serum was diluted with mobile phase including 100 or 500 mM TBA ion, the steroid sulfates in serum were retained in the pre-column; however, the steroid sulfates from the same sample diluted with mobile phase containing 5 mM TBA ion were not retained in the pre-column. Addition of an excess amount of counter ion (TBA ion) into the serum sample made it possible to retain the steroid sulfates in the pre-column. This method was applied to column-switching HPLC for measurement of steroid sulfates in serum using a semi-microcolumn as the analytical column.
- 公益社団法人日本薬学会の論文
- 1998-11-15
著者
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Yasui Koichiro
Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine Graduate School O
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Yasui Kohichiroh
Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine Graduate School O
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Fujinami A
Clinical Chemistry Laboratory Kobe Pharmaceutical University
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Kobayashi Yoshiharu
Tokyo University of Pharmacy and Life Science
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Kobayashi Y
Faculty Of Pharmaceutical Sciences Teikyo University Sagamiko
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Kobayashi Yoshiharu
Clinical Chemistry And Medicinal Chemistry Laboratories Kobe Women's College Of Pharmacy
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TAGAWA Noriko
Clinical Chemistry Laboratory
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Tagawa Noriko
神戸薬科大学 病態生化学研究室
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TSURUTA Hisao
Analytical Chemistry Research Laboratry, Irika Instruments Inc.,
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FUJINAMI Aya
Clinical Chemistry Laboratory, Kobe Pharmaceutical University
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Tsuruta Hisao
Analytical Chemistry Research Laboratry Irika Instruments Inc.
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Yoshida Kyotaro
Dep. Of Medical Biochemistry Kobe Pharmaceutical Univ.
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