Simultaneous Analysis of Benzphetamine and Its Metabolites, and Quantitation of Urinary p-Hydroxy-N-benzylamphetamine by Micellar Electrokinetic Chromatography
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概要
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We developed a method for simultaneous analysis of benzphetamine (BZ) and its metabolites, p-hydroxy-N-benzylamphetamine (pHBA), p-hydroxybenzphetamine (pHBZ), amphetamine (AP), methamphetamine and p-hydroxymethamphetamine by micellar electrokinetic chromatography (MEKC). Urine samples from 0-15 h (3h) intervals) after oral administration of BZ (10 mg) were hydrolyzed with β-glucuronidase (EC 3.2.1.31) at 37℃ overnight. The treated urine was applied to a solid phase extraction column Bond Elut Certify^<[○!R]>. After sequentially washing the column wiht water, 0.1 mol/l acetic acid and methanol, the samples were eluted with dichloromethane : isopropanol : 28% ammonium hydroxide=78.4 : 19.6 : 2.0 (v/v %). The eluate was evaporated and the residue dissolved in running buffer was analyzed by MEKC. In urine from 0-3 h, AP, pHBZ and pHBA were detected. After that, only pHBA, which is one of the major metabolites of BZ in human urine, could be detected in the urine by the present method. A method for quantitation of pHBA by MEKC is described here. The effects of acetonitrile and sodium dodecyl sulfate in the running buffer of MEKC on the separation of BZ and its metabolites are also reported.
- 公益社団法人日本薬学会の論文
- 1998-11-15
著者
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Yasui Koichiro
Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine Graduate School O
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Yasui Kohichiroh
Molecular Gastroenterology And Hepatology Kyoto Prefectural University Of Medicine Graduate School O
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MIYAZAWA Tadashi
Forensic Science Laboratory, Osaka Prefecture Police Headquarters
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Fujinami A
Clinical Chemistry Laboratory Kobe Pharmaceutical University
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Miyazawa T
Forensic Science Laboratory Osaka Prefectural Police Headquarters
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Miyazawa Tadashi
Forensic Science Laboratory Osaka Prefecture Police Headquarters
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Kobayashi Yoshiharu
Tokyo University of Pharmacy and Life Science
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Kobayashi Y
Faculty Of Pharmaceutical Sciences Teikyo University Sagamiko
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Kobayashi Yoshiharu
Clinical Chemistry And Medicinal Chemistry Laboratories Kobe Women's College Of Pharmacy
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TAGAWA Noriko
Clinical Chemistry Laboratory
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Tagawa Noriko
神戸薬科大学 病態生化学研究室
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FUJINAMI Aya
Clinical Chemistry Laboratory, Kobe Pharmaceutical University
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Yoshida Kyotaro
Dep. Of Medical Biochemistry Kobe Pharmaceutical Univ.
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