Glycyrrhetinic Acid Induced Apoptosis in Murine Splenocytes
スポンサーリンク
概要
- 論文の詳細を見る
Glycyrrhetinic acid (GA) is known to inhibit glucocorticoid metabolism inhibiting 11β-hydroxysteroid dehydrogenase (11β-HSD). Moreover, GA administration to mice has been shown to affect the lymphoid organs through elevation of endogenous corticosterone concentration. The effect of GA administration on thymus has been demonstrated to show that considerable amounts of thymocytes undergo apoptosis by elevated levels of corticosterone in systemic circulation. However, the effect of GA administration on peripheral lymphocytes has remained unknown. In our current study, we demonstrated that a significant involution of spleen as well as thymus occurred within 24h of a single administration of GA in mice. In addition, a flow cytometric analysis of the splenocytes taken from mice treated with GA showed a significant increase in the number of apoptotic cells which exhibited translocated phosphatidylserine outside the plasma membrane. Furthermore, considerable inhibition of 11β-HSD activity in GA-treated mice was observed in liver and spleen, resulting in a significant increase in concentration of corticosterone in the blood. These facts showed that the apoptosis of splenocytes was the result of indirect effect of GA through elevated levels of corticosterone. We confirmed this using cultured splenocytes in vitro where no apoptotic effect of GA was observed. We concluded that GA administration induces cell death of not only thymocytes that are naive to corticosterone, but also splenocytes that are usually stable to its physiological concentrations.
- 公益社団法人日本薬学会の論文
- 2001-01-01
著者
-
HIRANO Toshihiko
Department of Clinical Pharmacology, Tokyo University of Pharmacy and Life Sciences
-
OKA Kitaro
Department of Clinical Pharmacology, Tokyo University of Pharmacy and Life Sciences
-
Hirano Tomohisa
Graduate School Of Pharmaceutical Sciences The University Of Tokyo
-
Hirano Toshihiko
Tokyo Univ. Medical Univ. Pharmacy And Life Sci. Tokyo Jpn
-
HOMMA Masato
Department of Pharmaceutical Sciences, Graduate School of Comprehensive Human Sciences, University o
-
Hirano Toshihiko
Department Of Clinical Pharmacology Tokyo University Of Pharmacy And Life Science
-
Oka Kitaro
Department Of Clinical Pharmacology School Of Pharmacy Tokyo University Of Pharmacy And Life Science
-
Oka Kitaro
Department Of Clinical Pharmacology Tokyo University Of Pharmacy And Life Science
-
Oka Kitaro
School Of Pharmacy Tokyo University Of Pharmacy And Life Science
-
Hirano Toshihiko
Department Of Clinical Pharmacology School Of Pharmacy Tokyo University Of Pharmacy And Life Science
-
Oka K
Department Of Clinical Pharmacology School Of Pharmacy Tokyo University Of Pharmacy And Life Science
-
Homma Masato
Department Of Clinical Pharmacology School Of Pharmacy Tokyo University Of Pharmacy And Life Science
-
Homma Masato
Department Of Clinical Pharmacology Institute Of Cliniical Medicine University Of Tsukuba
-
HORIGOME Hiroshi
Department of Clinical Pharmacology, School of Pharmacy, Tokyo University of Pharmacy and Life Scien
-
Horigome Hiroshi
Department Of Clinical Pharmacology School Of Pharmacy Tokyo University Of Pharmacy And Life Science
-
Hirano T
Department Of Clinical Pharmacology Tokyo University Of Pharmacy And Life Science
関連論文
- Irinotecan Hydrochloride (CPT-11) in Dialysis Patients with Gastrointestinal Cancer
- Evidence of Different Pharmacokinetics Including Relationship among AUC, Peak, and Trough Levels between Cyclosporine and Tacrolimus in Renal Transplant Recipients Using New Pharmacokinetic Parameter : Why Cyclosporine Is Monitored by C_2 Level and Tacrol
- Evidence of Different Pharmacokinetics Including Relationship among AUC, Peak, and Trough Levels between Cyclosporine and Tacrolimus in Renal Transplant Recipients Using New Pharmacokinetic Parameter : Why Cyclosporine Is Monitored by C_2 Level and Tacrol
- Evidence of Different Pharmacokinetics Including Relationship among AUC, Peak, and Trough Levels between Cyclosporine and Tacrolimus in Renal Transplant Recipients Using New Pharmacokinetic Parameter : Why Cyclosporine Is Monitored by C_2 Level and Tacrol
- Evidence of Different Pharmacokinetics Including Relationship among AUC, Peak, and Trough Levels between Cyclosporine and Tacrolimus in Renal Transplant Recipients Using New Pharmacokinetic Parameter : Why Cyclosporine Is Monitored by C_2 Level and Tacrol
- High Performance Liquid Chromatography for Routine Monitoring of Serum Flecainide
- MDR1 Gene Polymorphism in Japanese Patients with Schizophrenia and Mood Disorders Including Depression(Biopharmacy)
- Effect of Rabeprazole on MDR1-Mediated Transport of Rhodamine 123 in Caco-2 and Hvr100-6 Cells(Biopharmacy)
- A New Cytotoxic Cholestane Bisdesmoside from Ornithogalum saundersiae Bulbs
- Mechanism of Superoxide Dismutase-Like Activity of Fe(II) and Fe(III) Complexes of Tetrakis-N, N, N', N' (2-pyridylmethyl)ethylenediamine
- EFFICIENT SUPEROXIDE DISMUTASE ACTIVITIES OF NOVEL IRON COMPLEXES
- PJ-213 Relationship Between the Activity of p450-CYP2D6 and Serum Level of Flecainide(Arrhythmia, Pharmacological Therapy 3 (A) : PJ36)(Poster Session (Japanese))
- P-Glycoprotein Function in Peripheral Blood Mononuclear Cells of Myasthenia Gravis Patients Treated with Tacrolimus(Pharmacology)
- Use of Sample Hematocrit Value to Correct Blood Tacrolimus Concentration Derived by Microparticle Enzyme Immunoassay(Highlighted paper selected by Editor-in-chief,Miscellaneous)
- Sedation and Plasma Concentration of Clonidine Hydrochloride for Pre-anesthetic Medication in Pediatric Surgery
- Induction of Apoptosis in Human Lung Fibroblasts and Peripheral Lymphocytes in Vitro by Shosaiko-to Derived Phenolic Metabolites
- P-Glycoprotein Functions in Peripheral-Blood CD4^+ Cells of Patients with Systemic Lupus Erythematosus(Pharmacology)
- Liquid Chromatographic Determination of Plasma Ropivacaine for Assessing Pharmacokinetics of the Viscous Preparation(Biopharmacy)
- Glycyrrhetinic Acid Induced Apoptosis in Murine Splenocytes
- Metabolization of Flecainide is Affected by Gender (Arrhythmia, Pharmacological Therapy 6 (A), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- A Novel Fluorescent Probe for Zinc Ion Based on Boron Dipyrromethene (BODIPY) Chromophore
- Pharmacodynamic Parameters of Immunosuppressive Drugs Are Not Correlated with Age, Duration of Dialysis, Percentage of Lymphocytes or Lymphocyte Stimulation Index in Renal Transplant Recipients(Pharmacology)
- False Positive Blood Tacrolimus Concentration in Microparticle Enzyme Immunoassay
- Assessment of Clonidine Orally Disintegrating Tablet for Pre-anesthetic Medication in Pediatric Surgery(Biopharmacy)
- Differential mRNA Expression of Inflammatory Cytokines in Cultured Human Fetal Membrane Cells Responding to Influenza Virus Infection
- Apoptosis in Cultured Human Fetal Membrane Cells Infected with Influenza Virus
- Improvement of Separation Method of Fragmented DNA from an Apoptotic Cell DNA Sample for the Quantitation Using Agarose Gel Electrophoresis
- Characteristics of Delayed Excretion of Flavonoids in Human Urine after Administration of Shosaiko-to, a Herbal Medicine
- Suppression of Apoptotic Cell Death Progressed in Vitro with Incubation of the Chorion Laeve Tissues of Human Fetal Membrane by Glucocorticoid
- Stereochemistry and Putative Origins of Flavanones Found in Post-administration Urine of the Traditional Chinese Remedies Shosaiko-to and Daisaiko-to
- Chromatographic identification of phenolic compounds in human urine following oral administration of the herbal medicines Daisaiko-to and Shosaiko-to
- Extrashot-ODS, a syringe-type minicolumn sample injector for a reversed-phase high-performance liquid chromatographic column Application to antiepileptics in human sera
- Methoxy- and Fluoro-chalcone Derivatives Arrest Cell Cycle Progression and Induce Apoptosis in Human Melanoma Cell A375
- Morphological Transformation and Phosphatidylserine Exposure in Erythrocytes Treated with Ribavirin
- Central retinal vein occlusion in hypertensive patients with chronic hepatitis C treated with interferon alpha and ribavirin
- Quantification of the Levels of CYP2D6 mRNA in Peripheral Blood Leukocytes by Reverse Transcriptase Polymerase Chain Reaction HPLC
- Erythrocyte ribavirin concentration for assessing hemoglobin reduction in interferon and ribavirin combination therapy
- Cerebral Blood Flow Measurement of Severely Head-Injured Patients during Mild Hypothermia
- Steroid Withdrawal Based on Lymphocyte Sensitivity to Endogenous Steroid in Renal Transplant Recipients
- Antiproliferative and Apoptosis-Inducing Effects of Lipophilic Vitamins on Human Melanoma A375 Cells in Vitro
- Impact of solute carrier family 29 member 1 (SLC29A1) single nucleotide polymorphisms on mRNA expression in peripheral blood mononuclear cells
- Platelet-derived adenosine 5'-triphosphate suppresses activation of human hepatic stellate cell : In vitro study