Suppression of Apoptotic Cell Death Progressed in Vitro with Incubation of the Chorion Laeve Tissues of Human Fetal Membrane by Glucocorticoid
スポンサーリンク
概要
- 論文の詳細を見る
In the trophoblast layer of the chorion laeve of human fetal membranes obtaiend by cesarean section at the month of normal parturition, cells with condensed nuclei could be observed by histochemical examination. Incubating fetal membranes at 37℃ in vitro in cultivation medium, the frequency of cells with condensed nuclei increased in the chorion laeve, associating with an increase in DNA fragmentation and the population of in situ TUNEL (terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-X nick end labeling) staining-positive cells. The progressed apoptotic cell death in the chorion laeve in vitro was suppressed by incubation of the tissue in the presence of glucocorticoids, cortisone or hydrocortisone, which was also demonstrated by DNA fragmentation analysis and in situ TUNEL staining. These results reveal that a substantial proportion of trophoblast cells in the chorion laeve of human fetal membranes are induced to undergo apoptosis at the end of pregnancy, and that the apoptosis progresses rapidly in vitro as the incubation period increases. It is suggested that certain hormones such as glucocorticoid, may be related to the regulation of the apoptosis in human fetal membranes.
- 公益社団法人日本薬学会の論文
- 1998-10-15
著者
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Oka Kitaro
Department Of Clinical Pharmacology Tokyo University Of Pharmacy And Life Science
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Oka Kitaro
School Of Pharmacy Tokyo University Of Pharmacy And Life Science
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Oka Kitaro
Clinical Phamacology Department Of Biochemistry Tokyo University Of Pharmacy And Life Science
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OHYAMA Kunio
Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy & Life
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Oka K
Department Of Clinical Pharmacology School Of Pharmacy Tokyo University Of Pharmacy And Life Science
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Ohyama Kunio
Department Of Biochemistry Faculty Of Pharmacy Tokyo University Of Pharmacy & Life Science
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Suga Tetsuya
Clinical Biochemistry Department Of Biochemistry Tokyo University Of Pharmacy And Life Science
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Hirakawa Shun
The First Department of Obstetrics and Gynecology,School of Medicine,Toho University
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Hirakawa S
The First Department Of Obstetrics And Gynecology School Of Medicine Toho University
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Hirakawa Shun
The First Department Of Obstetrics And Gynecology School Of Medicine Toho University
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BESSHO Toshio
Yoneyama Maternity Hospital
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YAMAKAWA Toshio
Department of Biochemistry, School of Pharmacy, Tokyo University ofPharmacy and Life Science
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EMURA Ayako
Department of Biochemistry, Department of Biochemistry, Tokyo University of Pharmacy and Life Scienc
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TAMURA Hiroshi
Clinical Biochemistry, Tokyo University of Pharmacy and Life Science
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山川 富雄
Department Of Biochemistry Faculty Of Pharmacy Tokyo University Of Pharmacy & Life Science
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山川 富雄
Research Laboratories Nippon Chemiphar Co. Ltd.
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Tamura H
Clinical Biochemistry Tokyo University Of Pharmacy And Life Science
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Emura A
Department Of Biochemistry Department Of Biochemistry Tokyo University Of Pharmacy And Life Science
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Yamakawa Toshio
Department Of Biochemistry School Of Pharmacy Tokyo University Of Pharmacy & Life Science (forme
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OHYAMA Kunio
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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