Predominant Contribution of IFN-β Expression to Apoptosis Induction in Human Uterine Cervical Fibroblast Cells by Influenza-Virus Infection(Biochemistry/Molecular Biology)
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概要
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We have been investigating an apoptosis induction in human fetal membrane cells by influenza virus (IV) infection and the contribution of apoptosis induction to the viral infection-defense response between a fetus and the maternal body. For studying any role of uterine cells in the anti-viral response, we investigated the molecular mechanism of the apoptotic induction in human uterine cervical fibroblast cell line (HCF) by IV infection. IV type A and B infection induced DNA fragmentation in HCF. In IV-infected HCF, gene mRNA expression levels of interleukine (IL)-1β, IL-6, tumor necrosis factor (TNF) α, Fas ligand, interferon regulatory factor (IRF)-1, interferon (IFN) α and IFN β increased as compared with those in mock treatment cells, and the induction of mRNAs for double stranded RNA dependent protein kinase (PKR), indolamine 2,3-deoxygenase (IDO) and 2'-5' oligoadenylate synthetase (2-5 OAS) were indicated, which had a role for a host defense response induced by IFN-β. The amount of IFN-β protein increased by IV-infection, and DNA fragmentation was inhibited with anti-IFN-β antibody and PKR inhibitor (2-aminopurine). Furthermore, a synthetic double stranded RNA, poly I:C, could induce almost the same phenomena as that induced by virus infection. We conclude that IV-infection induces the apoptosis in HCF cells through the IFN-β expression regulated by double stranded RNA and IRF-1 induction, and suggest that the IFN-β induction may be the predominant contribution to the IV infection induced HCF apoptosis.
- 社団法人日本薬学会の論文
- 2004-11-01
著者
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Toyoda Hiroo
Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life
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Toyoda Hiroo
Department Of Clinical Molecular Genetics Faculty Of Pharmacy Tokyo University Of Pharmacy & Lif
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OHYAMA Kunio
Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy & Life
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SANO Tomomi
Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy & Life
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Sano Tomomi
Department Of Biochemistry School Of Pharmacy Tokyo University Of Pharmacy And Life Science
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Sano Tomomi
Department Of Clinical Molecular Genetics Faculty Of Pharmacy Tokyo University Of Pharmacy & Lif
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Ohyama K
Department Of Clinical Molecular Genetics Faculty Of Pharmacy Tokyo University Of Pharmacy & Lif
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Ohyama Kunio
Department Of Biochemistry Faculty Of Pharmacy Tokyo University Of Pharmacy & Life Science
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OHYAMA Kunio
Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture
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