Sialyl Lewis X-Carboxymethylpullulan Conjugate : A Novel Homing Device to Spleen and Lymph Nodes(Biopharmacy)

概要

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We have previously found that carboxymethylpullulan (CMPul) conjugated with sialyl Lewis X (Neu5Acα2-3Galβ1-4(Fucα1-3)GlcNAc-, 2-3SLe^χ) preferentially accumulates in the lymph nodes and spleen. In the present study, we investigated the structural requirements of the 2-3SLe^χ moiety for this accumulation using rats. Radiolabeled CMPul conjugates with various degrees of substitution (d.s.) of the 2-3SLe^χ moiety were intravenously administered to rats, and their tissue distributions were monitored by radioactivity. When the d.s. was more than 0.5, preferential accumulation in the lymph nodes as well as the spleen was observed. However, when the d.s. was 0.025, little effect of the 2-3SLe^χ moiety was noted. Changes in the carbohydrate structure of 2-3SLe^χ, i.e., a change to α2-6-linked sialic acid (Neu5Acα2-6Gal/β-4(Fucal-3)GlcNAc-, 2-6SLe^χ) or an elimination of the fucose (Neu5Acα2-3Gal/β1-4GlcNAc-, sialyl N-acethyllactosamine (SLN)), also made the 2-3SLe^χ moiety ineffective. Furthermore, Microautoradiography analyses revealed that 2-3SLe^χ-CMPul was incorporated by particular subsets of macrophages in these tissues, and that CMPul and SLN-CMPul were also located in the same cells to a lesser extent. 2-3SLe^χ-CMPul may be able to serve as a novel drug delivery carrier to target drugs to the peripheral lymphoid tissues.
公益社団法人日本薬学会の論文
2004-08-01