Autoradiographic Studies on the Binding Sites of ^<125>I-Adrenomedullin in Rat Tissues
スポンサーリンク
概要
- 論文の詳細を見る
Adrenomedullin (AM) is a novel hypotensive peptide isolated from the human pheochromocytoma. In the present study, ^<125>I-AM was intravenously administered to rats and its distribution was examined by autoradiographic (ARG) techniques using wholebody, light microscopic (LM) and electron microscopic (EM) methods. Whole-body-ARG after administration of ^<125>I-AM showed the highest radioactivity in the lung (paren-chyma), followed by the renal cortex and the liver. When localization of ^<125>I-AM in the lung was examined by LM-ARG, silver grains were localized over the arteries, capillaries and venules. In the arteries, the endothelial cells of the small arteries (lumen diameter 20-50μm) were preferentially labelled and there was little labelling on the large arteries. There were few silver grains on the smooth muscle cells along the entire pulmonary vasculature system. EM-ARG showed many silver grains on the endothelial cells of capillaries and venules. Very few silver grains were observed on the lung when an excess amount of unlabelled AM was administered with ^<125>I-AM. These results indicated that ^<125>I-AM specific binding sites are most abundant in the lung, and the major binding sites are on the endothelial cells of small arteries, alveolar capillaries and venules.
- 日本組織細胞化学会の論文
著者
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長谷川 浩
金沢大学理工研究域物質化学系
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Kitamura Kazuo
First Department of Internal Medicine, University of Miyazaki
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Eto Tanenao
First Department Of Internal Medicine Faculty Of Medicine University Of Miyazaki
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KANGAWA KENJI
National Cardiovascular Center Research Institute
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Mizuhira Vinci
東京医科歯科大学難治疾患研究所
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Mizuhira V
Medical Research Institute Tokyo Medical And Dental University
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Kangawa Kenji
National Cardiovascular Center
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MIZOJIRI Kenji
Developmental Research Laboratories, Shionogi & Co., Ltd.
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HASEGAWA Hiroshi
Developmental Research Laboratories, Shionogi & Co., Ltd.
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MATSUO Hisayuki
National Cardiovascular Center Research Institute
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Notoya Mitsuru
Developmental Research Laboratories, Shionogi & Co., Ltd.
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Mizuhira Vinci
Developmental Research Laboratories, Shionogi & Co. Ltd.
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Inazawa Kazuhiro
Developmental Research Laboratories, Shionogi & Co. Ltd.
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Matsuo Hisayuki
Department Of Internal Medicine Circulatory And Body Fluid Regulation Faculty Of Medicine University
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Hasegawa H
Laboratory Of Structure And Function Research Tokai University School Of Medicine
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Notoya Mitsuru
Developmental Research Laboratories Shionogi & Co. Ltd.
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Mizojiri Kenji
Developmental Research Laboratories Shionogi & Co. Ltd.
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Eto Tanenao
First Department Of Internal Medicine Department Of Pathology Miyazaki Medical College
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Notoya M
Shionogi & Co. Ltd. Toyonaka Jpn
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Kitamura Kazuo
First Department Of Internal Medicine Miyazaki Medical College University Of Miyazaki
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Inazawa Kazuhiro
Developmental Research Laboratories Shionogi & Co. Ltd.
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Kitamura Kazuo
First Department Of Internal Medicine Miyazaki Medical College
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Hasegawa Hiroshi
Developmental Research Laboratories Shionogi & Co. Ltd.
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Mizojiri Kenji
Developmental Research Laboratories, Shionogi & Co. Ltd.
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