NEW TISSUE FIXATION METHOD FOR CYTOCHEMISTRY BY THE AID OF MICROWAVE IRRADIATION : II DETAILS (<Symposium>Microwave Fixation in Histochemistry)
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概要
- 論文の詳細を見る
Microwave irradiation (MWI) was evaluated as a means of fixation for the detection of enzymatic activities such as alkaline phosphatase, acid phosphatase, and glucose-6-phosphatase in the liver and the kidney. MWI enabled the detection of intracellular alkaline phosphatase enzymatic activities in perinuclear cisternae and rough endoplasmic reticulum cisternae of the hepatocytes; this had not been possible with the conventionally fixed cells. With MWI, acid phosphatase tended to be diffusible from lysosomes. This suggested that microwave energy had some effect on the membrane.We tested MWI fixation also for autoradiographic studies using ^3H-thymidine and ^<125>I-peptide. Excellent ultrastructural preservation and clear detection of radiolabelled compounds showed the MWI technique to be excellent and reliable for autoradiographic studies.
- 日本組織細胞化学会の論文
著者
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長谷川 浩
金沢大学理工研究域物質化学系
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Mizuhira Vinci
東京医科歯科大学難治疾患研究所
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Mizuhira V
Medical Research Institute Tokyo Medical And Dental University
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NOTOYA Mitsuru
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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HASEGAWA Hiroshi
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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MIZUHIRA Vinci
Shionogi Research Laboratories, Shionogi & Co., Ltd.
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Hasegawa H
Laboratory Of Structure And Function Research Tokai University School Of Medicine
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Mizuhira Vinci
Shionogi Co. Ltd.
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Notoya Mitsuru
Shionogi Research Laboratories Shionogi & Co .ltd.
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Notoya M
Shionogi & Co. Ltd. Toyonaka Jpn
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