Overexpression of Glyceraldehyde-3-phospahe Dehydrogenase Is Not Involved in 5-Hydroxytryptamine (5-HT)-Induced Necrosis in Cultured Cerebrocortical Neurons(Pharmacology)

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概要

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• Cerebrocortical cell cultures were prepared from 1-d-old rats. On post-culture day 6, 5-hydroxytryptamine (5-HT) was added to the medium and cells were exposed for another 3 d. 5-HT elicited cytotoxicity in a dose-dependent manner, and the survival rate of neuronal cells was decreased to 64.9±5.0% at 0.1 mM concentration. Chromatin staining with Hoechst 33258 and electron microscopy revealed that the 5-HT-induced neuronal death was entirely due to necrosis. Pretreatments with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antisense oligonucleotide and several classical apoptotic inhibitors did not exhibit neuroprotection in this paradigm. Northern blot analysis showed that the enhancement of GAPDH mRNA levels was undetected during cell death. The present results demonstrate that GAPDH overexpression is not involved in the 5-HT-induced necrotic death pathway.

• 公益社団法人日本薬学会の論文
• 2004-08-01

著者

• Tanaka Muneo

  Research Center Shiseido Ltd.

• Tanaka M

  Faculty Of Pharmaceutical Sciences Tokushima Bunri University

• 田中 誠

  School Of Pharmaceutical Sciences Kitasato University

• Sunaga K

  Group On Cellular Neurobiology Josai University

• Sunaga Katsuyoshi

  Group On Neuropharmacology Faculty Of Pharmaceutical Sciences

• Sunaga Katsuyoshi

  Group Of Biochemical Pharmacology Faculty Of Pharmaceutical Sciences Josai University

• TAJIMA Hisao

  Discovery Research Laboratories I, Minase Institute, Ono Pharmaceutical Co., Ltd.

• TANAKA Masaharu

  Safety Research Laboratories, Fukui Institute, Ono Pharmaceutical Co., Ltd.

• KUWAE Toyoyasu

  Group on Cellular Neurobiology, Josai University

• KATSUBE Nobuo

  Safety Research Laboratories, Fukui Institute, Ono Pharmaceutical Co., Ltd.

• Tajima Hisao

  Discovery Research Laboratories I Minase Institute Ono Pharmaceutical Co. Ltd.

• Katsube N

  Fukui Safety Research Laboratories Ono Pharmaceutical Co. Ltd.

• Kuwae T

  Group On Cellular Neurobiology Josai University

• Tanaka Masaharu

  Safety Research Laboratories Fukui Institute Ono Pharmaceutical Co. Ltd.

• SUNAGA Katsuyoshi

  Group of Bichemical Phamacology, Faculty of Pharmaceutical Scuebces, Josai University

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