EXAMINATION OF BIAL'S REACTION, WHICH IS A HISTOCHEMICAL DEMONSTRATION METHOD OF SIALIC ACID
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概要
- 論文の詳細を見る
Various methods have been introduced for chemical detection of sialic acid. Among those methods we applied resorcinol reaction, orcinol reaction (Bial) and thiobarbituric acid reaction to histochemical study as they appeared to be useful. As the result, it was found that only orcinol reaction (Bial) shows reaction on specimen. When orcinol reaction was conducted by using pure reagents, fructose, sorbose, tryptophan and sialic acid (N-acetylneuraminic acid) indicated positive reaction. Fructose and sorbose were discriminated from sialic acid because of their color and its quick fading. Discrimination of tryptophan from sialic acid was possible by tryptophan staining by Serra's method and tetrazonium method as its reaction part is different. It was also possible to discriminate these substances by spectrophotomter and thin-layer chromatography. It was therefore concluded that the substance which develops color in Bial's reaction on specimen is most probably sialic acid.
- 日本組織細胞化学会の論文
著者
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KIKKAWA NOBUTERU
Department of Surgery, Osaka National Hospital
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HIROSE Masaaki
Department of Physics, Tokyo Metropolitan University
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Razzaq A.
The 2nd Department Of .surgery Osaka University Medical School
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MURAI NOBUHIRO
The 2nd Department of Surgery, Osaka University Medical School
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ASO REIZO
The 2nd Department of .Surgery, Osaka University Medical School
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KIKKAWA NOBUTERU
The 2nd Department of .Surgery, Osaka University Medical School
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HIROSE SHUNTA
Department of Surgery, Osaka University Medical School
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YASUTOMI MASAYUKI
Department of Surgery, Osaka University Medical School
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MURAI NOBUHIRO
Department of Surgery, Osaka University Medical School
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IWASA ZENJI
Department of Surgery, Osaka University Medical School
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ASO REIZO
Department of Surgery, Osaka University Medical School
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RAZZAQ A.
Department of Surgery, Osaka University Medical School
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SINDO KATSUHISA
Department of Surgery, Osaka University Medical School
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Hirose Masaaki
The 2nd Department Of .surgery Osaka University Medical School
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Aso Reizo
The 2nd Department Of .surgery Osaka University Medical School
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Iwasa Zenji
The First Department Of Surgery Kinki University School Of Medicine
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Hirose Shunta
First Department Of Surgery Kinki University School Of Medicine
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Hirose Shunta
Department Of Surgery Osaka University Medical School
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Murai Nobuhiro
The 2nd Department Of Surgery Osaka University Medical School
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Hirose Masaaki
Department Of Physics Tokyo Metropolitan University
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Sindo Katsuhisa
The 2nd Department Of .surgery Osaka University Medical School
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Yasutomi Masayuki
Department Of Surgery Kinki University School Of Medicine
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Shibata Hideo
First Department Of Surgery Kinki University School Of Medicine
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Hirose Syunta
Department Of Surgery Osaka University Medical School
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Shimomukai Hiroshi
First Department Of Surgery Kinki University School Of Medicine
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Yasutomi Masayuki
The 2nd Department Of Surgery Osaka University Medical School
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Kikkawa Nobuteru
The 2nd Department Of .surgery Osaka University Medical School
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Kikkawa Nobuteru
Department Of Surgery Minoh City Hospital
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Yasutomi Masayuki
The First Department Of Surgery Kinki University School Of Medicine
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Iwasa Zenji
Department Of Surgery Osaka University Medical School
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Yasutomi Masayuki
Department Of Surgery Osaka University Medical School
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Hirose Masaaki
Department of Food Science and Nutrition, Nara Womens University
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