N-(2-アミノエチル)-N-(2-ヒドロキシエチル)ジチオカルバミン酸を用いる銅(II)の吸光光度定量

概要

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The N-(2-aminoethyl)-N-(2-hydroxyethyl) dithiocar-bamic acid (Hahdtc) reacts with copper(II) and forms a water-soluble complex having an absorption maximum at 432nm. The absorbance of the complex at the wave-length is maximum and constant when the pH of the sample is in the range of 3.5 and 7.6, moreover, at pH 4.5, it does not change for 45min. Beer's law holds for (3.21〜102.6)μg of copper (II) in the sample of 25-ml volume with the molar absorption coefficient ε_<432>=(1.29±0.02)×10^4cm^<-1> mol^<-1> 1, being Sandell's sensitivity (4.9±0.6)×10^<-3>μgcm^<-2>. The recommended procedure is as follows. To (3.21〜102.6)μg of copper in a 25-ml volumetric flask are added 5ml of 2mM Hahdtc solution and 5ml of acetate buffer solution (pH 4.5). The mixture is flled up to 25ml with water. The absorbance measurement is carried out at 432nm against water within 30min after mixing. Less than 10mg Al(III), Ca(II), Gd(II), Mg(II), Mn(II) and Zn(II), less than 1mg Pb(II) and less than 0.5mg Cr(III) do not interfere. Interference by 1mg of Bi(III),Co(II) and Ni(II) is masked by the addition of Na_2H_2edta. Less than 10mg Fe(III) is masked by reduction with ascorbic acid. Less than 0.2mg Hg(II) does not interfere, but when Na_2H_2edta coexists, it interferes seriously.
社団法人日本分析化学会の論文
1978-01-05