イオン交換クロマトグラフィーにおける段数とひずみ率に及ぼす溶離液の濃度の影響

概要

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Copper(II) ion mounted on the column of Amberlite IR-120, 400600 mesh, 43 mm in length, is eluted by aqueous HNO3. The eluted Cu(II) is determined polarographically after the manual fractionation of the eluant. From the elution curves, as shown in Fig. 2, the plate number n and the skew ratio S, proposed by Cruickshank and Everette and defined by the equation(12), are evaluated. It is found that, with the decreasing concentration of HNO3, the peak width Wh becomes broader and the retention volume VR increases. The plots of log VR and log Wh against the logarithm of HNO3 concentration give two straight lines, respectively. It is found further that there is an optimum HNO3 concentration showing the highest plate number, and that at this optimum concentration the skew ratio also approaches most closely to unity, namely, the peak shows the highest symmetry.
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