薄層けい光デンシトメトリーによるノスカピンの定量

概要

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Noscapine gave fluorescence on a silica gel plate by heating at 120℃. This fluorescence reaction was used for the determination of noscapine in pharmaceutical preparations. Various for the determination were examined, and the following procedure was recommended. Procedure: To a portion of the finely sample, equivalent to about 0.4 mg of noscapine, added 2 ml of 0.1 N hydrochloric acid and the resultant mixture is shaked. After addition of 1 ml of 10% ammonia solution, noscapine is extracted with 5.0 ml of benzene. Five μl of the benzene solution is spotted on a Kieselgel 60 pre-coated plate. The plate is developed with chloroform-benzene-acetone (3 : 3 : 1). After developing, the plate is heated for 5 hours at 120℃. The fluorescence intensity of the spot is measured at excitation 365 nm and emission 450 nm with a fluorescence densitometer (Shimadzu two wavelength chromatoscaner CS-900). The content of each packs in commercial preparations was between 96.3% and 105.1%. The proposed procedure is very simple and applicable to the content uniformity test of pharmaceutical preparations containing noscapine.
社団法人日本分析化学会の論文
1977-11-05